ZFIN ID: ZDB-LAB-970424-122
Cheng Lab
PI/Director: Cheng, Keith C.
Contact Person: Cheng, Keith C.
Email: kcheng@psu.edu
URL: http://www.pennstatehershey.org/web/pathology/keith-cheng
Address: The Jake Gittlen Cancer Research Institute Hershey Medical Center Pennsylvania State University College of Medicine 500 University Drive Hershey, PA 17033 USA
Country: United States
Phone: (717) 531-4704
Fax: (717) 531-5634
Line Designation: kc

Show all 20 genomic features

Our laboratory is using genetics to dissect vertebrate mechanisms of mutation and cell differentiation, using the zebrafish as a model system. We used parthenogenetic screens to generate genomic instabilty mutants using a mosaic eye screen and cell differentiation mutants using histological arrays (manuscripts in preparation). We hypothesize that some of our mutants will be tumor suppressor genes.

Sinha, Soniya Graduate Student Thomas, Georgia Graduate Student Hubley, Peggy Research Staff
Mest, Jason Research Staff Rush, Lindsay Research Staff Sabaliauskas, Nicole Research Staff
Wentz, Christina Research Staff Lighty, Melissa Fish Facility Staff

Katz, S.R., Yakovlev, M.A., Vanselow, D.J., Ding, Y., Lin, A.Y., Parkinson, D.Y., Wang, Y., Canfield, V.A., Ang, K.C., Cheng, K.C. (2021) Whole-organism 3D quantitative characterization of zebrafish melanin by silver deposition micro-CT. eLIFE. 10:
Yang, H., Luan, Y., Liu, T., Lee, H.J., Fang, L., Wang, Y., Wang, X., Zhang, B., Jin, Q., Ang, K.C., Xing, X., Wang, J., Xu, J., Song, F., Sriranga, I., Khunsriraksakul, C., Salameh, T., Li, D., Choudhary, M.N.K., Topczewski, J., Wang, K., Gerhard, G.S., Hardison, R.C., Wang, T., Cheng, K.C., Yue, F. (2020) A map of cis-regulatory elements and 3D genome structures in zebrafish. Nature. 588(7837):337-343
Van Nuffel, S., Ang, K.C., Lin, A.Y., Cheng, K.C. (2020) Chemical Imaging of Retinal Pigment Epithelium in Frozen Sections of Zebrafish Larvae Using ToF-SIMS. Journal of the American Society for Mass Spectrometry. 32(1):255-261
Ding, Y., Vanselow, D.J., Yakovlev, M.A., Katz, S.R., Lin, A.Y., Clark, D.P., Vargas, P., Xin, X., Copper, J.E., Canfield, V.A., Ang, K.C., Wang, Y., Xiao, X., De Carlo, F., van Rossum, D.B., La Riviere, P., Cheng, K. (2019) Computational 3D histological phenotyping of whole zebrafish by X-ray histotomography. eLIFE. 8:
Lin, A.Y., Ding, Y., Vanselow, D.J., Katz, S.R., Yakovlev, M.A., Clark, D.P., Mandrell, D., Copper, J.E., van Rossum, D.B., Cheng, K.C. (2018) Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography. Journal of visualized experiments : JoVE. (140)
Copper, J.E., Budgeon, L.R., Foutz, C.A., van Rossum, D.B., Vanselow, D.J., Hubley, M.J., Clark, D.P., Mandrell, D.T., Cheng, K.C. (2017) Comparative analysis of fixation and embedding techniques for optimized histological preparation of zebrafish. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 208:38-46
Xin, X., Clark, D., Ang, K.C., van Rossum, D.B., Copper, J., Xiao, X., La Riviere, P.J., Cheng, K.C. (2017) Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. Proceedings of SPIE--the International Society for Optical Engineering. 10060:
Ding, Y., Tavolara, T., Cheng, K. (2016) Automated Detection of Retinal Cell Nuclei in 3D Micro-CT Images of Zebrafish using Support Vector Machine Classification. Proceedings of SPIE--the International Society for Optical Engineering. 9791:
Cheng, K.C., Katz, S.R., Lin, A.Y., Xin, X., Ding, Y. (2016) Whole-Organism Cellular Pathology: A Systems Approach to Phenomics. Advances in genetics. 95:89-115
Thomas, G.K. (2015) A molecular and systems biology analysis of pleiotropic vertebrate phenotypes. Ph.D. Thesis. :218
Tsetskhladze, Z.R., Canfield, V.A., Ang, K.C., Wentzel, S.M., Reid, K.P., Berg, A.S., Johnson, S.L., Kawakami, K., and Cheng, K.C. (2012) Functional assessment of human coding mutations affecting skin pigmentation using zebrafish. PLoS One. 7(10):e47398
Cheng, K.C., Xin, X., Clark, D.P., and La Riviere, P. (2011) Whole-animal imaging, gene function, and the Zebrafish Phenome Project. Current opinion in genetics & development. 21(5):620-9
Canada, B.A., Thomas, G.K., Cheng, K.C., and Wang, J.Z. (2011) SHIRAZ: an automated histology image annotation system for zebrafish phenomics. Multimedia Tools and Applications. 51(2):401-440
Ekker, S.C., Parichy, D.M., and Cheng, K.C. (2008) Research implications of pigment biology in zebrafish. Zebrafish. 5(4):233-235
Aleström, P., Begemann, G., Carvan, M.J. 3rd, Cheng, K.C., Crosier, K., Crosier, P., Ekker, S., Huttenlocher, A., Kawakami, K., Kelly, G., Korzh, V., Lieschke, G., Mione, M., Neely, M.N., Neuhauss, S., and Trede, N.S. (2008) Views on four key questions about zebrafish research. Zebrafish. 5(1):9-24
Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D. (2007) Expression of the G protein gammaT1 subunit during zebrafish development. Gene expression patterns : GEP. 7(5):574-583
Moore, J.L., Rush, L.M., Breneman, C., Mohideen, M.A., and Cheng, K.C. (2006) Zebrafish Genomic Instability Mutants and Cancer Susceptibility. Genetics. 174(2):585-600
Leung, T., Chen, H., Stauffer, A.M., Giger, K.E., Sinha, S., Horstick, E.J., Humbert, J.E., Hansen, C.A., and Robishaw, J.D. (2006) Zebrafish G protein γ2 is required for VEGF signaling during angiogenesis. Blood. 108(1):160-166
Sabaliauskas, N.A., Foutz, C.A., Mest, J.R., Budgeon, L.R., Sidor, AT, Gershenson, J.A., Joshi, S.B., and Cheng, K.C. (2006) High-throughput zebrafish histology. Methods (San Diego, Calif.). 39(3):246-254
Blasiole, B., Canfield, V.A., Vollrath, M.A., Huss, D., Mohideen, M.A., Dickman, J.D., Cheng, K.C., Fekete, D.M., and Levenson, R. (2006) Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish. Developmental Biology. 294(1):148-160
Tang, J., Hu, G., Hanai, J.I., Yadlapalli, G., Lin, Y., Zhang, B., Galloway, J., Bahary, N., Sinha, S., Thisse, B., Thisse, C., Jin, J.P., Zon, L.I., and Sukhatme, V.P. (2006) A critical role for calponin 2 in vascular development. The Journal of biological chemistry. 281(10):6664-6672
Canada, B.A., Cheng, K.C., and Wang, J.Z. (2006) QCHARM: A Novel Computational and Scientific Visualization Framework for Facilitating Discovery and Improving Diagnostic Reliability in Medicine. AMIA Annual Symposium proceedings. :870
Lamason, R.L., Mohideen, M.A., Mest, J.R., Wong, A.C., Norton, H.L., Aros, M.C., Jurynec, M.J., Mao, X., Humphreville, V.R., Humbert, J.E., Sinha, S., Moore, J.L., Jagadeeswaran, P., Zhao, W., Ning, G., Makalowska, I., McKeigue, P.M., O'donnell, D., Kittles, R., Parra, E.J., Mangini, N.J., Grunwald, D.J., Shriver, M.D., Canfield, V.A., and Cheng, K.C. (2005) SLC24A5, a putative cation exchanger, affects pigmentation in zebrafish and humans. Science (New York, N.Y.). 310(5755):1782-1786
Croushore, J.A., Blasiole, B., Riddle, R.C., Thisse, C., Thisse, B., Canfield, V.A., Robertson, G.P., Cheng, K.C., and Levenson, R. (2005) Ptena and ptenb genes play distinct roles in zebrafish embryogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 234(4):911-921
Duffy, K.T., McAleer, M.F., Davidson, W.R., Kari, L., Kari, C., Liu, C.G., Farber, S.A., Cheng, K.C., Mest, J.R., Wickstrom, E., Dicker, A.P., and Rodeck, U. (2005) Coordinate control of cell cycle regulatory genes in zebrafish development tested by cyclin D1 knockdown with morpholino phosphorodiamidates and hydroxyprolyl-phosphono peptide nucleic acids. Nucleic acids research. 33(15):4914-4921
Chan, B., Sinha, S., Cho, D., Ramchandran, R., and Sukhatme, V.P. (2005) Critical roles of CD146 in zebrafish vascular development. Developmental Dynamics : an official publication of the American Association of Anatomists. 232(1):232-244
Gerhard, G.S., Malek, R.L., Keller, E., Murtha, J., and Cheng, K.C. (2004) Zebrafish, Killifish, Neither Fish, Both Fish?. The journals of gerontology. Series A, Biological sciences and medical sciences. 59(9):B873-B875
Moore, J.L., Gestl, E.E., and Cheng, K.C.. (2004) Mosaic eyes, genomic instability mutants, and cancer susceptibility. The Zebrafish: Cellular and Developmental Biology,2nd Ed. Methods Cell Biol.. 76:555-568
Cheng, K.C., Levenson, R., and Robishaw, J.D. (2003) Functional genomic dissection of multimeric protein families in zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(3):555-567
Mohideen, M.A., Beckwith, L.G., Tsao-Wu, G.S., Moore, J.L., Wong, A.C., Chinoy, M.R., and Cheng, K.C. (2003) Histology-based screen for zebrafish mutants with abnormal cell differentiation. Developmental Dynamics : an official publication of the American Association of Anatomists. 228(3):414-423
Sinha, S. (2003) An in-situ hybridization study of g-protein gamma subunits in the developing zebrafish. Master's Thesis. :88P
Gerhard, G.S. and Cheng, K.C. (2002) A call to fins! Zebrafish as a gerontological model. Aging Cell. 1(2):104-111
Gerhard, G., Kauffman, E., Wang, X., Stewart, R., Moore, J., Kasales, C., Demidenko, E., and Cheng, K. (2002) Life spans and senescent phenotypes in two strains of zebrafish (Danio rerio). Experimental gerontology. 37(8-9):1055-1068
Clarke, L.E., Cheng, K., Beckwith, L.G., Moore, J.L., Wang, X., and Boyer, P.J. (2002) Expression in adult zebrafish of antigens detected by immunomarkers commonly used in diagnostic pathology laboratories: Prelude to evaluation of spontaneously arising and induced neoplasms. Journal of neuropathology and experimental neurology. 61(5):112
Moore, J.L., Aros, M., Steudel, K.G., and Cheng, K.C. (2002) Fixation and decalcification of adult zebrafish for histological, immunocytochemical, and genotypic analysis. Biotechniques. 32(2):296-298
Rajarao, S.J., Canfield, V.A., Mohideen, M.A., Yan, Y.-L., Postlethwait, J.H., Cheng, K.C., and Levenson, R. (2001) The repertoire of Na,K-ATPase alpha and beta subunit genes expressed in the zebrafish, Danio rerio. Genome research. 11(7):1211-1220
Mohideen, M.A.P.K., Moore, J.L., and Cheng, K.C. (2000) Centromere-linked microsatellite markers for linkage groups 3, 4, 6, 7, 13, and 20 of zebrafish (Danio rerio). Genomics. 67(1):102-106
Beckwith, L.G., Moore, J.L., Tsao-Wu, G.S., Harshbarger, J.C., and Cheng, K.C. (2000) Ethylnitrosourea induces neoplasia in zebrafish (Danio rerio). Laboratory investigation; a journal of technical methods and pathology. 80(3):379-385
Tsao-Wu, G.S., Weber, C.H., Budgeon, L.R., and Cheng, K.C. (1998) Agarose-embedded tissue arrays for histologic and genetic analysis. Biotechniques. 25:614-618
Cheng, K.C. and Moore, J.L. (1997) Genetic dissection of vertebrate processes in zebrafish: A comparison of uniparental and two-generation screens. Biochemistry and cell biology = Biochimie et biologie cellulaire. 75:525-533
Gestl, E.E., Kauffman, E.J., Moore, J.L., and Cheng, K.C. (1997) New conditions for generation of gynogenetic half-tetrad embryos in the zebrafish (Danio rerio). The Journal of heredity. 88(1):76-79
Kauffman, E.J., Gestl, E.E., Kim, D.J., Walker, C., Hite, J.M., Yan, G., Rogan, P.K., Johnson, S.L., and Cheng, K.C. (1995) Microsatellite-centromere mapping in the zebrafish (Danio rerio). Genomics. 30:337-341
Gestl, E.E. and Cheng, K.C. (1995) A small zebrafish genetics facility. Zebrafish Science Monitor. 3:6-8
Ham, P.H. and Cheng, K.C. (1995) Construction of zebrafish spawning cages. Zebrafish Science Monitor. 3:5
Cheng, K.C., E.J. Kauffman, and E. Gestl (1993) Tidbits (nibbles) on raising zebrafish babies. Zebrafish Science Monitor. 2:3,6