ZFIN ID: ZDB-LAB-201006-2
Drerup Lab
PI/Director: Drerup, Katie (Catherine)
Contact Person: Drerup, Katie (Catherine)
Email: drerup@wisc.edu
URL: http://dreruplab.com
Address: University of Wisconsin-Madison Department of Integrative Biology 250 N. Mills St. Rm 124/128 Madison, WI 53706
Country: United States
Line Designation: uw


In neurons, retrograde transport is the process by which cargos, including trophic factors, organelles, cytoskeletal elements, and many others are carried toward microtubule minus ends in axons. During axon development, retrograde transport of signaling endosomes is essential for axon outgrowth. Similarly, retrograde transport of injury signals has been linked to degenerative events, tying retrograde axonal transport to establishment, maintenance, and regeneration of neural circuits. Retrograde axonal transport is accomplished primarily by the single Cytoplasmic dynein 1 motor protein complex. Perhaps not surprisingly, defects in dynein-dependent cargo transport in neurons have been associated with neurodegenerative diseases ranging from Charcot-Marie-Tooth disease to Parkinson’s and Amyotrophic Lateral Sclerosis. Therefore, there is great interest in understanding how this motor binds cargos and moves them at the right time to the right place.

To better understand dynein function, we have developed novel tools and techniques to address the mechanisms of retrograde cargo transport. First, we optimized techniques to image axonal transport and microtubule dynamics in vivo using the zebrafish. Second, we designed a forward genetic screen to identify adaptor proteins for dynein-dependent cargo transport. Third, we optimized in vivo functional imaging to determine how disruption of cargo transport effects neural circuit function. Together, we are using these tools and techniques to work further our understanding of the cellular and molecular mechanisms governing retrograde transport and how disrupting this process impacts neural circuit structure and function.

Wong, Hiu-Tung Candy Post-Doc Chlebowski, Mady Research Staff

Mandal, A., Wong, H.C., Pinter, K., Mosqueda, N., Beirl, A., Lomash, R.M., Won, S., Kindt, K.S., Drerup, C.M. (2020) Retrograde mitochondrial transport is essential for organelle distribution and health in zebrafish neurons. The Journal of neuroscience : the official journal of the Society for Neuroscience. 41(7):1371-1392
Spinner, M.A., Pinter, K., Drerup, C.M., Herman, T.G. (2020) A Conserved Role for Vezatin Proteins in Cargo-Specific Regulation of Retrograde Axonal Transport. Genetics. 216(2):431-445
Wong, H.C., Zhang, Q., Beirl, A.J., Petralia, R.S., Wang, Y.X., Kindt, K. (2019) Synaptic mitochondria regulate hair-cell synapse size and function. eLIFE. 8:
Liao, Y.C., Fernandopulle, M.S., Wang, G., Choi, H., Hao, L., Drerup, C.M., Patel, R., Qamar, S., Nixon-Abell, J., Shen, Y., Meadows, W., Vendruscolo, M., Knowles, T.P.J., Nelson, M., Czekalska, M.A., Musteikyte, G., Gachechiladze, M.A., Stephens, C.A., Pasolli, H.A., Forrest, L.R., St George-Hyslop, P., Lippincott-Schwartz, J., Ward, M.E. (2019) RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether. Cell. 179:147-164.e20
Tuttle, A., Drerup, C.M., Marra, M., McGraw, H., Nechiporuk, A.V. (2019) Retrograde Ret signaling controls sensory pioneer axon outgrowth. eLIFE. 8:
Mandal, A., Pinter, K., Drerup, C.M. (2018) Analyzing Neuronal Mitochondria in vivo Using Fluorescent Reporters in Zebrafish. Frontiers in cell and developmental biology. 6:144
Zhang, Q., Li, S., Wong, H.C., He, X.J., Beirl, A., Petralia, R.S., Wang, Y.X., Kindt, K.S. (2018) Synaptically silent sensory hair cells in zebrafish are recruited after damage. Nature communications. 9:1388
Herbert, A.L., Fu, M.M., Drerup, C.M., Gray, R.S., Harty, B.L., Ackerman, S.D., O'Reilly-Pol, T., Johnson, S.L., Nechiporuk, A.V., Barres, B.A., Monk, K.R. (2017) Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.. Proceedings of the National Academy of Sciences of the United States of America. 114:E9153-E9162
Drerup, C.M., Herbert, A.L., Monk, K.R., Nechiporuk, A.V. (2017) Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons. eLIFE. 6
Drerup, C.M., Lusk, S., Nechiporuk, A. (2016) Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones. The Journal of neuroscience : the official journal of the Society for Neuroscience. 36:7014-26
Drerup, C.M., Nechiporuk, A.V. (2016) In vivo analysis of axonal transport in zebrafish. Methods in cell biology. 131:311-29
Zhang, Q.X., He, X.J., Wong, H.C., Kindt, K.S. (2016) Functional calcium imaging in zebrafish lateral-line hair cells. Methods in cell biology. 133:229-252
Drerup, C.M., and Nechiporuk, A.V. (2013) JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes. PLoS Genetics. 9(2):e1003303
Drerup, C.M., Wiora, H.M., and Morris, J.A. (2010) Characterization of the overlapping expression patterns of the zebrafish LIS1 orthologs. Gene expression patterns : GEP. 10(1):75-85
Drerup, C.M., Ahlgren, S.C., and Morris, J.A. (2007) Expression profiles of ndel1a and ndel1b, two orthologs of the NudE-Like gene, in the zebrafish. Gene expression patterns : GEP. 7(6):672-679