Community Action Needed: Please respond to the NIH RFI
ZFIN ID: ZDB-LAB-130206-1
Besharse Lab
PI/Director: Besharse, Joseph C.
Contact Person: Volberding, Peter
Address: Dept of Cell Biology, Neurobiology and Anatomy Medical College of Wisconsin 8701 Watertown Plank Road Milwaukee, WI 53226-0509
Country: United States
Phone: 414-955-8188
Fax: 414-955-6517
Line Designation: mw

Show all 11 genomic features

The Besharse laboratory is interested in the cell biology of trafficking, ciliogenesis and microtubule-based motors, particularly in photoreceptors. Photoreceptors are particularly interesting because the light sensing organelle is an elaborate sensory cilium. Fundamental mechanisms central to ciliogenesis such as intraflagellar transport are essential for building the light sensing organelle. Similar processes are also essential throughout the life of the cell to support extensive turnover of photosensitive membranes. The laboratory uses both mice and zebrafish as animals models. Transgenic zebrafish are particularly useful for studying photoreceptor trafficking events that are essential for vision.

Bader, Jason Post-Doc Fogerty, Joseph Graduate Student Lewis, Tylor Graduate Student
Volberding, Peter Technical Staff

Song, P., Fogerty, J., Cianciolo, L.T., Stupay, R., Perkins, B.D. (2020) Cone Photoreceptor Degeneration and Neuroinflammation in the Zebrafish Bardet-Biedl Syndrome 2 (bbs2) Mutant Does Not Lead to Retinal Regeneration. Frontiers in cell and developmental biology. 8:578528
Solanki, A.K., Kondkar, A.A., Fogerty, J., Su, Y., Kim, S.H., Lipschutz, J.H., Nihalani, D., Perkins, B.D., Lobo, G.P. (2020) A Functional Binding Domain in the Rbpr2 Receptor Is Required for Vitamin A Transport, Ocular Retinoid Homeostasis, and Photoreceptor Cell Survival in Zebrafish. Cells. 9(5):
Fogerty, J., Stepanyan, R., Cianciolo, L.T., Tooke, B.P., Perkins, B.D. (2019) Genomic non-redundancy of the mir-183/96/182 cluster and its requirement for hair cell maintenance. Scientific Reports. 9:10302
Lessieur, E.M., Song, P., Nivar, G.C., Piccillo, E.M., Fogerty, J., Rozic, R., Perkins, B.D. (2019) Ciliary genes arl13b, ahi1 and cc2d2a differentially modify expression of visual acuity phenotypes but do not enhance retinal degeneration due to mutation of cep290 in zebrafish. PLoS One. 14:e0213960
Lewis, T.R., Kundinger, S.R., Link, B.A., Insinna, C., Besharse, J.C. (2018) Kif17 phosphorylation regulates photoreceptor outer segment turnover. BMC Cell Biology. 19:25
Lewis, T.R., Zareba, M., Link, B.A., Besharse, J.C. (2017) Cone myoid elongation involves unidirectional microtubule movement mediated by dynein-1. Molecular biology of the cell. 29(2):180-190
Lewis, T.R., Kundinger, S.R., Pavlovich, A.L., Bostrom, J.R., Link, B.A., Besharse, J.C. (2017) Cos2/Kif7 and OSM-3/Kif17 Regulate Onset of Outer Segment Development in Zebrafish Photoreceptors Through Distinct Mechanisms. Developmental Biology. 425(2):176-190
Lessieur, E.M., Fogerty, J., Gaivin, R.J., Song, P., Perkins, B.D. (2017) The Ciliopathy Gene ahi1 Is Required for Zebrafish Cone Photoreceptor Outer Segment Morphogenesis and Survival. Investigative ophthalmology & visual science. 58:448-460
Bell, B.A., Yuan, A., Dicicco, R.M., Fogerty, J., Lessieur, E.M., Perkins, B.D. (2016) The adult zebrafish retina: In vivo optical sectioning with Confocal Scanning Laser Ophthalmoscopy and Spectral-Domain Optical Coherence Tomography. Experimental Eye Research. 153:65-78
Collery, R.F., Volberding, P.J., Bostrom, J.R., Link, B.A., Besharse, J.C. (2016) Loss of Zebrafish Mfrp Causes Nanophthalmia, Hyperopia, and Accumulation of Subretinal Macrophages. Investigative ophthalmology & visual science. 57:6805-6814
Song, P., Dudinsky, L., Fogerty, J., Gaivin, R., Perkins, B.D. (2016) Arl13b Interacts With Vangl2 to Regulate Cilia and Photoreceptor Outer Segment Length in Zebrafish. Investigative ophthalmology & visual science. 57:4517-4526
Fogerty, J., Denton, K., Perkins, B.D. (2016) Mutations in the Dynein1 Complex are Permissible for Basal Body Migration in Photoreceptors but Alter Rab6 Localization. Advances in experimental medicine and biology. 854:209-15
Miesfeld, J.B., Gestri, G., Clark, B.S., Flinn, M.A., Poole, R.J., Bader, J.R., Besharse, J.C., Wilson, S.W., Link, B.A. (2015) Yap and Taz regulate retinal pigment epithelial cell fate. Development (Cambridge, England). 142(17):3021-32