ZFIN Lab: Ramchandran Lab

ZFIN ID: ZDB-LAB-100630-1

Ramchandran Lab

PI/Director:	Ramchandran, Ramani
Contact Person:	Leigh, Noah
Email:	nleigh@mcw.edu
URL:
Address:	C3420, 8401 Watertown Plank Road Milwaukee, 53226 USA
Country:	United States
Phone:	414-955-2397
Fax:	414-955-6325
Line Designation:	mw

GENOMIC FEATURES ORIGINATING FROM THIS LAB

Show all 7 genomic features

STATEMENT OF RESEARCH INTERESTS

The Ramchandran Lab is interested in studying mechanisms related to vasculogenesis and angiogenesis during early embryonic development. We are also interested in developing zebrafish as a model system for target-based small molecule screening.

LAB MEMBERS

Li, Keguo Post-Doc	Schupp, Marcus Post-Doc	Sinha, Indranil Post-Doc
Chun, Chang Zoon Research Staff	Horswill, Mark Research Staff	Leigh, Noah Research Staff
Samant, Ganesh Research Staff

ZEBRAFISH PUBLICATIONS OF LAB MEMBERS

Zakaria, Z.Z., Eisa-Beygi, S., Benslimane, F.M., Ramchandran, R., Yalcin, H.C. (2022) Design and Microinjection of Morpholino Antisense Oligonucleotides and mRNA into Zebrafish Embryos to Elucidate Specific Gene Function in Heart Development. Journal of visualized experiments : JoVE. (186)

Thirugnanam, K., Prabhudesai, S., Van Why, E., Pan, A., Gupta, A., Foreman, K., Zennadi, R., Rarick, K.R., Nauli, S.M., Palecek, S.P., Ramchandran, R. (2022) Ciliogenesis mechanisms mediated by PAK2-ARL13B signaling in brain endothelial cells is responsible for vascular stability. Biochemical pharmacology. 202:115143

Subramaniam, S., Liu, J., Fletcher, C., Ramchandran, R., Weiler, H. (2022) Coagulation Factor IIIa (f3a) Knockdown in Zebrafish Leads to Defective Angiogenesis and Mild Bleeding Phenotype. Frontiers in cell and developmental biology. 10:852989

Gupta, A., Thirugnanam, K., Thamilarasan, M., Mohieldin, A.M., Zedan, H.T., Prabhudesai, S., Griffin, M.R., Spearman, A.D., Pan, A., Palecek, S.P., Yalcin, H.C., Nauli, S.M., Rarick, K.R., Zennadi, R., Ramchandran, R. (2022) Cilia proteins are biomarkers of altered flow in the vasculature. JCI insight. 7(6):

Gronseth, E., Gupta, A., Koceja, C., Kumar, S., Kutty, R.G., Rarick, K., Wang, L., Ramchandran, R. (2020) Astrocytes influence medulloblastoma phenotypes and CD133 surface expression. PLoS One. 15:e0235852

Dey, A., Prabhudesai, S., Zhang, Y., Rao, G., Thirugnanam, K., Hossen, M.N., Dwivedi, S.K.D., Ramchandran, R., Mukherjee, P., Bhattacharya, R. (2020) Cystathione β-synthase regulates HIF-1α stability through persulfidation of PHD2. Science advances. 6(27):

Chowdhury, T.A., Li, K., Ramchandran, R. (2019) Lessons learned from a lncRNA odyssey for two genes with vascular functions, DLL4 and TIE1. Vascular Pharmacology. 114:103-109

Eisa-Beygi, S., Benslimane, F.M., El-Rass, S., Prabhudesai, S., Abdelrasoul, M.K.A., Simpson, P.M., Yalcin, H.C., Burrows, P.E., Ramchandran, R. (2018) Characterization of Endothelial Cilia Distribution During Cerebral-Vascular Development in Zebrafish ( Danio rerio). Arteriosclerosis, Thrombosis, and Vascular Biology. 38:2806-2818

Prabhudesai, S., Koceja, C., Dey, A., Eisa-Beygi, S., Leigh, N.R., Bhattacharya, R., Mukherjee, P., Ramchandran, R. (2018) Corrigendum: Cystathionine β-Synthase Is Necessary for Axis Development in vivo.. Frontiers in cell and developmental biology. 6:121

Chowdhury, T.A., Koceja, C., Eisa-Beygi, S., Kleinstiver, B.P., Kumar, S.N., Lin, C.W., Li, K., Prabhudesai, S., Joung, J.K., Ramchandran, R. (2018) Temporal and Spatial Post-Transcriptional Regulation of Zebrafish tie1 mRNA by Long Noncoding RNA During Brain Vascular Assembly.. Arteriosclerosis, Thrombosis, and Vascular Biology. 38(7):1562-1575

Prabhudesai, S., Koceja, C., Dey, A., Eisa-Beygi, S., Leigh, N.R., Bhattacharya, R., Mukherjee, P., Ramchandran, R. (2018) Cystathionine β-Synthase Is Necessary for Axis Development in Vivo.. Frontiers in cell and developmental biology. 6:14

Jia, M., Andreassen, T., Jensen, L., Bathen, T.F., Sinha, I., Gao, H., Zhao, C., Haldosén, L.A., Cao, Y., Girnita, L., Moestue, S.A., Dahlman-Wright, K. (2016) Estrogen receptor α promotes breast cancer by reprogramming choline metabolism. Cancer research. 76(19):5634-5646

Rana, U., Liu, Z., Kumar, S.N., Zhao, B., Hu, W., Bordas, M., Cossette, S., Szabo, S., Foeckler, J., Weiler, H., Chrzanowska-Wodnicka, M., Holtz, M.L., Misra, R.P., Salato, V., North, P., Ramchandran, R., Miao, Q.R. (2016) Nogo-B receptor deficiency causes cerebral vasculature defects during embryonic development in mice. Developmental Biology. 410(2):190-201

Chun, C., Wu, Y., Lee, S.H., Williamson, E.A., Reinert, B.L., Jaiswal, A.S., Nickoloff, J.A., Hromas, R.A. (2016) The homologous recombination component EEPD1 is required for genome stability in response to developmental stress of vertebrate embryogenesis. Cell cycle (Georgetown, Tex.). 15(7):957-62

Saha, S., Chakraborty, P.K., Xiong, X., Dwivedi, S.K., Mustafi, S.B., Leigh, N.R., Ramchandran, R., Mukherjee, P., Bhattacharya, R. (2016) Cystathionine β-synthase regulates endothelial function via protein S-sulfhydration. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 30(1):441-56

Hoeppner, L.H., Sinha, S., Wang, Y., Bhattacharya, R., Dutta, S., Gong, X., Bedell, V.M., Suresh, S., Chun, C.Z., Ramchandran, R., Ekker, S.C., Mukhopadhyay, D. (2015) RhoC maintains vascular homeostasis by regulating VEGF-induced signaling in endothelial cells. Journal of Cell Science. 128(19):3556-68

Schupp, M.O., Waas, M., Chun, C.Z., Ramchandran, R. (2014) Transcriptional inhibition of etv2 expression is essential for embryonic cardiac development. Developmental Biology. 393(1):71-83

Rajasimha, H.K., Shirol, P.B., Ramamoorthy, P., Hegde, M., Barde, S., Chandru, V., Ravinandan, M.E., Ramchandran, R., Haldar, K., Lin, J.C., Babar, I.A., Girisha, K.M., Srinivasan, S., Navaneetham, D., Battu, R., Devarakonda, R., Kini, U., Vijayachandra, K., Verma, I.C. (2014) Organization for rare diseases India (ORDI) - addressing the challenges and opportunities for the Indian rare diseases' community. Genetics research. 96:e009

Zhao, C., Qiao, Y., Jonsson, P., Wang, J., Xu, L., Rouhi, P., Sinha, I., Cao, Y., Williams, C., Dahlman-Wright, K. (2014) Genome-wide Profiling of AP-1–Regulated Transcription Provides Insights into the Invasiveness of Triple-Negative Breast Cancer. Cancer research. 74(14):3983-94

Zhou, X., Fan, L.X., Li, K., Ramchandran, R., Calvet, J.P., and Li, X. (2014) SIRT2 regulates ciliogenesis and contributes to abnormal centrosome amplification caused by loss of polycystin-1. Human molecular genetics. 23(6):1644-55

Leigh, N.R., Schupp, M.O., Li, K., Padmanabhan, V., Gastonguay, A., Wang, L., Chun, C.Z., Wilkinson, G.A., and Ramchandran, R. (2013) Mmp17b is essential for proper neural crest cell migration in vivo. PLoS One. 8(10):e76494

Kilari, S., Remadevi, I., Zhao, B., Pan, J., Miao, R., Ramchandran, R., North, P.E., You, M., Rahimi, N., and Wilkinson, G.A. (2013) Endothelial Cell-Specific Chemotaxis Receptor (ECSCR) enhances Vascular Endothelial Growth Factor (VEGF) Receptor-2/Kinase insert domain receptor (KDR) activation and promotes proteolysis of internalized KDR. The Journal of biological chemistry. 288(15):10265-10274

Patra, C.R., Kim, J.H., Pramanik, K., d'Uscio, L.V., Patra, S., Pal, K., Ramchandran, R., Strano, M.S., and Mukhopadhyay, D. (2011) Reactive Oxygen Species Driven Angiogenesis by Inorganic Nanorods. Nano Letters. 11(11):4932-8

Samant, G.V., Schupp, M., Francois, M., Moleri, S., Kothinti, R.K., Chun, C.Z., Sinha, I., Sellars, S., Leigh, N., Pramanik, K., Horswill, M.A., Remadevi, I., Li, K., Wilkinson, G.A., Tabatabai, N.M., Beltrame, M., Koopman, P., and Ramchandran, R. (2011) Sox factors transcriptionally regulate ROBO4 expression in developing vasculature in Zebrafish. The Journal of biological chemistry. 286(35):30740-7

Lakshmikanthan, S., Sobczak, M., Chun, C., Henschel, A., Dargatz, J., Ramchandran, R., and Chrzanowska-Wodnicka, M. (2011) Rap1 promotes VEGFR2 activation and angiogenesis by a mechanism involving integrin alpha v beta 3. Blood. 118(7):2015-26

Chun, C.Z., Remadevi, I., Schupp, M.O., Samant, G.V., Pramanik, K., Wilkinson, G.A., and Ramchandran, R. (2011) Fli+ etsrp+ Hemato-Vascular Progenitor Cells Proliferate at the Lateral Plate Mesoderm during Vasculogenesis in Zebrafish. PLoS One. 6(2):e14732

Zhao, B., Chun, C., Liu, Z., Horswill, M.A., Pramanik, K., Wilkinson, G.A., Ramchandran, R., and Miao, R.Q. (2010) Nogo-B receptor is essential for angiogenesis in zebrafish via Akt pathway. Blood. 116(24):5423-5433

Verma, A., Bhattacharya, R., Remadevi, I., Li, K., Pramanik, K., Samant, G.V., Horswill, M., Chun, C.Z., Zhao, B., Wang, E., Miao, R.Q., Mukhopadhyay, D., Ramchandran, R., and Wilkinson, G.A. (2010) Endothelial cell-specific chemotaxis receptor (ecscr) promotes angioblast migration during vasculogenesis and enhances VEGF receptor sensitivity. Blood. 115(22):4614-4622

Jia, Y., Wu, S.L., Isenberg, J.S., Dai, S., Sipes, J.M., Field, L., Zeng, B., Bandle, R.W., Ridnour, L.A., Wink, D.A., Ramchandran, R., Karger, B.L., and Roberts, D.D. (2010) Thiolutin inhibits endothelial cell adhesion by perturbing Hsp27 interactions with components of the actin and intermediate filament cytoskeleton. Cell stress & chaperones. 15(2):165-181

Li, K., Blum, Y., Verma, A., Liu, Z., Pramanik, K., Leigh, N.R., Chun, C.Z., Samant, G.V., Zhao, B., Garnaas, M.K., Horswill, M.A., Stanhope, S.A., North, P.E., Miao, R.Q., Wilkinson, G.A., Affolter, M., and Ramchandran, R. (2010) A non-coding antisense RNA in tie-1 locus regulates tie-1 function in vivo. Blood. 115(1):133-139

Kanungo, J., Zheng, Y.L., Amin, N.D., Kaur, S., Ramchandran, R., and Pant, H.C. (2009) Specific Inhibition of Cyclin-dependent Kinase 5 Activity Induces Motor Neuron Development in vivo. Biochemical and Biophysical Research Communications. 386(1):263-267

Chun, C.Z., Kaur, S., Samant, G.V., Wang, L., Pramanik, K., Garnaas, M.K., Li, K., Field, L., Mukhopadhyay, D., and Ramchandran, R. (2009) Snrk-1 is involved in multiple steps of angioblast development and acts via notch signaling pathway in artery-vein specification in vertebrates. Blood. 113(5):1192-1199

Pramanik, K., Chun, C.Z., Garnaas, M.K., Samant, G.V., Li, K., Horswill, M.A., North, P.E., and Ramchandran, R. (2009) Dusp-5 and Snrk-1 coordinately function during vascular development and disease. Blood. 113(5):1184-1191

Makky, K., Duvnjak, P., Pramanik, K., Ramchandran, R., and Mayer, A.N. (2008) A Whole-Animal Microplate Assay for Metabolic Rate Using Zebrafish. Journal of Biomolecular Screening. 13(10):960-967

Kaur, S., Samant, G.V., Pramanik, K., Loscombe, P.W., Pendrak, M.L., Roberts, D.D., and Ramchandran, R. (2008) Silencing Of Directional Migration In Roundabout4 Knockdown Endothelial Cells. BMC Cell Biology. 9:61

Garnaas, M.K., Moodie, K.L., Liu, M.L., Samant, G.V., Li, K., Marx, R., Baraban, J.M., Horowitz, A., and Ramchandran, R. (2008) Syx, a RhoA Guanine Exchange Factor, Is Essential for Angiogenesis In Vivo. Circulation research. 103(7):710-716

Kanungo, J., Li, B.S., Goswami, M., Zheng, Y.L., Ramchandran, R., and Pant, H.C. (2007) Cloning and characterization of zebrafish (Danio rerio) cyclin-dependent kinase 5. Neuroscience letters. 412(3):233-238

Chun, C.Z., and Chen, T.T. (2007) Microinjecting recombinant rainbow trout Ea4-peptide of pro-IGF-I into zebrafish embryos causes abnormal development in heart, red blood cells, and vasculature. Comparative biochemistry and physiology. Toxicology & pharmacology : CBP. 145(1):39-44

Kaur, S., Abu-Abab, M.S., Singla, S., Yeo, S.Y., and Ramchandran, R. (2007) Expression pattern for unc5b, an axon guidance gene in embryonic zebrafish development. Gene Expression. 13(6):321-327

Hu, G., Tang, J., Zhang, B., Lin, Y., Hanai, J.I., Galloway, J., Bedell, V., Bahary, N., Han, Z., Ramchandran, R., Thisse, B., Thisse, C., Zon, L.I., and Sukhatme, V.P. (2006) A novel endothelial-specific heat shock protein HspA12B is required in both zebrafish development and endothelial functions in vitro. Journal of Cell Science. 119(Pt 19):4117-4126

Chun, C.Z., Tsai, H.J., and Chen, T.T. (2006) Trout Ea4- or human Eb-peptide of pro-IGF-I disrupts heart, red blood cell, and vasculature development in zebrafish embryos. Molecular reproduction and development. 73(9):1112-1121

Kaur, S., Castellone, M.D., Bedell, V.M., Konar, M., Gutkind, J.S., and Ramchandran, R. (2006) Robo4 signaling in endothelial cells imply attraction guidance mechanisms. The Journal of biological chemistry. 281(16):11347-11356

Bedell, V.M., Yeo, S.Y., Park, K.W., Chung, J., Seth, P., Shivalingappa, V., Zhao, J., Obara, T., Sukhatme, V.P., Drummond, I.A., Li, D.Y., and Ramchandran, R. (2005) roundabout4 is essential for angiogenesis in vivo. Proceedings of the National Academy of Sciences of the United States of America. 102(18):6373-6378

Chan, B., Sinha, S., Cho, D., Ramchandran, R., and Sukhatme, V.P. (2005) Critical roles of CD146 in zebrafish vascular development. Developmental Dynamics : an official publication of the American Association of Anatomists. 232(1):232-244