ZFIN ID: ZDB-LAB-090811-1
Kucenas Lab
PI/Director: Kucenas, Sarah
Contact Person: Kucenas, Sarah
Email: sk4ub@virginia.edu
URL: http://www.virginia.edu/biology/faculty/kucenas.htm
Address: Regular Mail: Department of Biology University of Virginia Physical & Life Sciences Building, Rm 312 PO Box 400328 Charlottesville, VA 22904-4328 Express Mail: Department of Biology 409 McCormick Rd University of Virginia Charlottesville, VA 22904
Country: United States
Phone: (434) 982-5436
Fax: (434) 982-5626
Line Designation: uva

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The long-term goal of our research program is to elucidate the roles of peripheral and central glia and glial-glial interactions during nervous system development, maintenance and disease/injury. Using Danio rerio (zebrafish) as a model system (and to a lesser extent, mouse), we combine genetic and pharmacological perturbation, single cell manipulation, laser ablation/axotomy and in vivo, time-lapse imaging to directly and continuously observe glial cell origins, behaviors and interactions in an intact vertebrate.

Fontenas, Laura Post-Doc Ali, Maria Graduate Student Arena, Kim Graduate Student
Brown, Evan Graduate Student Garrett, Taylor Graduate Student Smith, Ashtyn Graduate Student
Latimer, Andrew Research Staff Tocke, Lori Fish Facility Staff

Wiltbank, A.T., Steisnon, E.R., Criswell, S.J., Piller, M., Kucenas, S. (2022) Cd59 and inflammation regulate Schwann cell development. eLIFE. 11:
Arena, K.A., Zhu, Y., Kucenas, S. (2022) Transforming growth factor-beta signaling modulates perineurial glial bridging following peripheral spinal motor nerve injury in zebrafish. Glia. 70(10):1826-1849
Brown, E.A., Kawakami, K., Kucenas, S. (2021) A novel gene trap line for visualization and manipulation of erbb3b+ neural crest and glial cells in zebrafish. Developmental Biology. 482:114-123
Ali, M.F., Latimer, A.J., Wang, Y., Hogenmiller, L., Fontenas, L., Isabella, A.J., Moens, C.B., Yu, G., Kucenas, S. (2021) Met is required for oligodendrocyte progenitor cell migration in Danio rerio. G3 (Bethesda). 11(10):
Morris, A.D., Kucenas, S. (2021) A Novel Lysolecithin Model for Visualizing Damage in vivo in the Larval Zebrafish Spinal Cord. Frontiers in cell and developmental biology. 9:654583
Piller, M., Werkman, I.L., Brown, E.A., Latimer, A.J., Kucenas, S. (2021) Glutamate signaling via the AMPAR subunit GluR4 regulates oligodendrocyte progenitor cell migration in the developing spinal cord. The Journal of neuroscience : the official journal of the Society for Neuroscience. 41(25):5353-5371
Fontenas, L., Kucenas, S. (2021) Spinal cord precursors utilize neural crest cell mechanisms to generate hybrid peripheral myelinating glia. eLIFE. 10:
Zhu, Y., Crowley, S.C., Latimer, A.J., Lewis, G.M., Nash, R., Kucenas, S. (2019) Migratory Neural Crest Cells Phagocytose Dead Cells in the Developing Nervous System. Cell. 179(1):74-89.e10
Mikdache, A., Fontenas, L., Albadri, S., Revenu, C., Loisel-Duwattez, J., Lesport, E., Degerny, C., Del Bene, F., Tawk, M. (2019) Elmo1 function, linked to Rac1 activity, regulates peripheral neuronal numbers and myelination in zebrafish. Cellular and molecular life sciences : CMLS. 77(1):161-177
Fontenas, L., Welsh, T.G., Piller, M., Coughenour, P., Gandhi, A.V., Prober, D.A., Kucenas, S. (2019) The Neuromodulator Adenosine Regulates Oligodendrocyte Migration at Motor Exit Point Transition Zones. Cell Reports. 27:115-128.e5
Morioka, S., Perry, J.S.A., Raymond, M.H., Medina, C.B., Zhu, Y., Zhao, L., Serbulea, V., Onengut-Gumuscu, S., Leitinger, N., Kucenas, S., Rathmell, J.C., Makowski, L., Ravichandran, K.S. (2018) Efferocytosis induces a novel SLC program to promote glucose uptake and lactate release. Nature. 563:714-718
Fontenas, L., Kucenas, S. (2018) Motor Exit Point (MEP) Glia: Novel Myelinating Glia That Bridge CNS and PNS Myelin. Frontiers in Cellular Neuroscience. 12:333
Soma, S., Latimer, A.J., Chun, H., Vicary, A.C., Timbalia, S.A., Boulet, A., Rahn, J.J., Chan, S.S.L., Leary, S.C., Kim, B.E., Gitlin, J.D., Gohil, V.M. (2018) Elesclomol restores mitochondrial function in genetic models of copper deficiency. Proceedings of the National Academy of Sciences of the United States of America. 115(32):8161-8166
Wang, Y., Ali, M., Wang, Y., Kucenas, S., Yu, G. (2018) DETECTION AND TRACKING OF MIGRATING OLIGODENDROCYTE PROGENITOR CELLS FROM IN VIVO FLUORESCENCE TIME-LAPSE IMAGING DATA. Proceedings. IEEE International Symposium on Biomedical Imaging. 2018:961-964
Morris, A.D., Erisir, A., Criswell, S.J., Kucenas, S. (2017) Transmission Electron Microscopy of Zebrafish Spinal Motor Nerve Roots. Developmental dynamics : an official publication of the American Association of Anatomists. 246(11):956-962
Fontenas, L., Kucenas, S. (2017) Livin' On The Edge: glia shape nervous system transition zones. Current opinion in neurobiology. 47:44-51
Morris, A.D., Lewis, G.M., Kucenas, S. (2017) Perineurial Glial Plasticity and the Role of TGF-β in the Development of the Blood-Nerve-Barrier. The Journal of neuroscience : the official journal of the Society for Neuroscience. 37(18):4790-4807
Smith, C.J., Wheeler, M.A., Marjoram, L., Bagnat, M., Deppmann, C.D., Kucenas, S. (2017) TNFa/TNFR2 signaling is required for glial ensheathment at the dorsal root entry zone. PLoS Genetics. 13:e1006712
Fontenas, L., De Santis, F., Di Donato, V., Degerny, C., Chambraud, B., Del Bene, F., Tawk, M. (2016) Neuronal Ndrg4 Is Essential for Nodes of Ranvier Organization in Zebrafish. PLoS Genetics. 12:e1006459
Johnson, K., Barragan, J., Bashiruddin, S., Smith, C.J., Tyrrell, C., Parsons, M.J., Doris, R., Kucenas, S., Downes, G.B., Velez, C.M., Schneider, C., Sakai, C., Pathak, N., Anderson, K., Stein, R., Devoto, S.H., Mumm, J.S., Barresi, M.J. (2016) Gfap-positive radial glial cells are an essential progenitor population for later-born neurons and glia in the zebrafish spinal cord. Glia. 64(7):1170-89
Smith, C.J., Johnson, K., Welsh, T.G., Barresi, M.J., Kucenas, S. (2016) Radial glia inhibit peripheral glial infiltration into the spinal cord at motor exit point transition zones. Glia. 64(7):1138-53
Wheeler, M.A., Smith, C.J., Ottolini, M., Barker, B.S., Purohit, A.M., Grippo, R.M., Gaykema, R.P., Spano, A.J., Beenhakker, M.P., Kucenas, S., Patel, M.K., Deppmann, C.D., Güler, A.D. (2016) Genetically targeted magnetic control of the nervous system. Nature Neuroscience. 19(5):756-61
Kucenas, S. (2015) Perineurial Glia. Cold Spring Harbor perspectives in biology. 7(6)
Smith, C.J., Morris, A.D., Welsh, T.G., Kucenas, S. (2014) Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone. PLoS Biology. 12:e1001961
Lewis, G.M., Kucenas, S. (2014) Perineurial Glia Are Essential for Motor Axon Regrowth following Nerve Injury. The Journal of neuroscience : the official journal of the Society for Neuroscience. 34:12762-77
Clark, J.K., O'Keefe, A., Mastracci, T.L., Sussel, L., Matise, M.P., Kucenas, S. (2014) Mammalian Nkx2.2(+) perineurial glia are essential for motor nerve development. Developmental dynamics : an official publication of the American Association of Anatomists. 243(9):1116-29
Laquérriere, A., Maluenda, J., Camus, A., Fontenas, L., Dieterich, K., Nolent, F., Zhou, J., Monnier, N., Latour, P., Gentil, D., Héron, D., Desguerres, I., Landrieu, P., Beneteau, C., Delaporte, B., Bellesme, C., Baumann, C., Capri, Y., Goldenberg, A., Lyonnet, S., Bonneau, D., Estournet, B., Quijano-Roy, S., Francannet, C., Odent, S., Saint-Frison, M.H., Sigaudy, S., Figarella-Branger, D., Gelot, A., Mussini, J.M., Lacroix, C., Drouin-Garraud, V., Malinge, M.C., Attié-Bitach, T., Bessieres, B., Bonniere, M., Encha-Razavi, F., Beaufrère, A.M., Khung-Savatovsky, S., Perez, M.J., Vasiljevic, A., Mercier, S., Roume, J., Trestard, L., Saugier-Veber, P., Cordier, M.P., Layet, V., Legendre, M., Vigouroux-Castera, A., Lunardi, J., Bayes, M., Jouk, P.S., Rigonnot, L., Granier, M., Sternberg, D., Warszawski, J., Gut, I., Gonzales, M., Tawk, M., and Melki, J. (2014) Mutations in CNTNAP1 and ADCY6 are responsible for severe arthrogryposis multiplex congenita with axoglial defects. Human molecular genetics. 23(9):2279-89
Lewis, G.M., and Kucenas, S. (2013) Motor Nerve Transection and Time-lapse Imaging of Glial Cell Behaviors in Live Zebrafish. Journal of visualized experiments : JoVE. (76)
Binari, L.A., Lewis, G.M., and Kucenas, S. (2013) Perineurial Glia Require Notch Signaling during Motor Nerve Development but Not Regeneration. The Journal of neuroscience : the official journal of the Society for Neuroscience. 33(10):4241-4252
Takada, N., Kucenas, S., and Appel, B. (2010) Sox10 is necessary for oligodendrocyte survival following axon wrapping. Glia. 58(8):996-1006
Latimer, A., and Jessen, J.R. (2010) Extracellular matrix assembly and organization during zebrafish gastrulation. Matrix biology : journal of the International Society for Matrix Biology. 29(2):89-96
Kucenas, S., Wang, W.D., Knapik, E.W., and Appel, B. (2009) A selective glial barrier at motor axon exit points prevents oligodendrocyte migration from the spinal cord. The Journal of neuroscience : the official journal of the Society for Neuroscience. 29(48):15187-15194
Kucenas, S., Cox, J.A., Soto, F., Lamora, A., and Voigt, M.M. (2009) Ectodermal P2X receptor function plays a pivotal role in craniofacial development of the zebrafish. Purinergic signalling. 5(3):395-407
Latimer, A.J., and Jessen, J.R. (2008) hgf/c-met expression and functional analysis during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 237(12):3904-3915
Coyle, R.C., Latimer, A., and Jessen, J.R. (2008) Membrane-type 1 matrix metalloproteinase regulates cell migration during zebrafish gastrulation: Evidence for an interaction with non-canonical Wnt signaling. Experimental cell research. 314(10):2150-2162
Kucenas, S., Snell, H., and Appel, B. (2008) nkx2.2a promotes specification and differentiation of a myelinating subset of oligodendrocyte lineage cells in zebrafish. Neuron glia biology. 4(2):71-81
Kucenas, S., Takada, N., Park, H.C., Woodruff, E., Broadie, K., and Appel, B. (2008) CNS-derived glia ensheath peripheral nerves and mediate motor root development. Nature Neuroscience. 11(2):143-151
Kirby, B.B., Takada, N., Latimer, A.J., Shin, J., Carney, T.J., Kelsh, R.N., and Appel, B. (2006) In vivo time-lapse imaging shows dynamic oligodendrocyte progenitor behavior during zebrafish development. Nature Neuroscience. 9(12):1506-1511
Latimer, A.J., and Appel, B. (2006) Notch signaling regulates midline cell specification and proliferation in zebrafish. Developmental Biology. 298(2):392-402
Kucenas, S., Soto, F., Cox, J.A., and Voigt, M.M. (2006) Selective labeling of central and peripheral sensory neurons in the developing zebrafish using P2X(3) receptor subunit transgenes. Neuroscience. 138(2):641-652
Cox, J.A., Kucenas, S., and Voigt, M.M. (2005) Molecular characterization and embryonic expression of the family of N-methyl-D-aspartate receptor subunit genes in the zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 234(3):756-766
Sarmah, B., Latimer, A.J., Appel, B., and Wente, S.R. (2005) Inositol polyphosphates regulate zebrafish left-right asymmetry. Developmental Cell. 9(1):133-145
Latimer, A.J., Shin, J., and Appel, B. (2005) her9 promotes floor plate development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 232(4):1098-1104
Kucenas, S.C. (2005) Zebrafish P2X receptors illuminate the way : shedding light on somatosensory and cranial neural crest mechanisms. Ph.D. Thesis. :123p
Latimer, A.J. (2005) Notch signaling regulates midline development in zebrafish. Ph.D. Thesis. :194P
Appel, B., Marasco, P., McClung, L.E., and Latimer, A.J. (2003) lunatic fringe regulates delta-notch induction of hypochord in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 228(2):281-286
Kucenas, S., Li, Z., Cox, J.A., Egan, T.M., and Voigt, M.M. (2003) Molecular characterization of the zebrafish P2X receptor subunit gene family. Neuroscience. 121(4):935-945
Latimer, A.J., Dong, X., Markov, Y., and Appel, B. (2002) Delta-Notch signaling induces hypochord development in zebrafish. Development (Cambridge, England). 129(11):2555-2563