ZFIN ID: ZDB-LAB-080414-1
Nechiporuk Lab
PI/Director: Nechiporuk, Alex
Contact Person: Nechiporuk, Alex
Email: nechipor@ohsu.edu
URL: http://www.ohsu.edu/academic/nechiporuk-lab/index.html
Address: OHSU School of Medicine Department of Cell and Developmental Biology 3181 SW Sam Jackson Park Rd Basic Sciences L215 Rm. 5369 (office) Rm. 5350 (lab) Portland, OR 97239
Country: United States
Phone: 503 494 1294
Fax: 503-494-4253
Line Designation: nl

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Our laboratory is interested in understanding how naïve progenitor cells segregate to give rise to diverse cell types that eventually form an organ. The question of organ formation is even more profound and challenging when applied to a vertebrate nervous system, where hundreds of cell types exist. We address this question in the context of cranial placodes, placode-derived sensory ganglia, and zebrafish lateral line system. The improper development of placode-derived sensory components of the peripheral nervous system, which are essential for the formation of cranial sensory systems such as somatosensation, and taste, have been implicated in many human disorders, including chronic obstructive pulmonary disease, migraines, bladder overactivity, erectile dysfunction, heart failure, arrhythmia, and others. Thus, uncovering genes that specify cranial placodes and ganglia should provide better understanding for the mechanisms underlying these disorders.

In addition to cranial placodes and ganglia, we also study development of mechanosensory organ system, called lateral line. The mechanosensory lateral line system of aquatic vertebrates is used to detect displacement of water and controls various types of swimming behavior. Lateral line provides an excellent system for studying basic biological processes, such as collective migration, cell specification, morphogenesis, patterning, and proliferation in the genetically-tractable model system such as zebrafish.

McGraw, Hillary Post-Doc Harding, Molly Graduate Student McCarroll, Matthew N. Graduate Student
Forbes, Austin Technical Staff

Olson, H.M., Nechiporuk, A.V. (2020) Lamellipodia-like protrusions and focal adhesions contribute to collective cell migration in zebrafish. Developmental Biology. 469:125-134
Jurynec, M.J., Bai, X., Bisgrove, B.W., Jackson, H., Nechiporuk, A., Palu, R.A.S., Grunwald, H.A., Su, Y.C., Hoshijima, K., Yost, H.J., Zon, L.I., Grunwald, D.J. (2019) The Paf1 Complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors. Development (Cambridge, England). 146(24):
McCarroll, M.N., Gendelev, L., Kinser, R., Taylor, J., Bruni, G., Myers-Turnbull, D., Helsell, C., Carbajal, A., Rinaldi, C., Kang, H.J., Gong, J.H., Sello, J.K., Tomita, S., Peterson, R.T., Keiser, M.J., Kokel, D. (2019) Zebrafish behavioural profiling identifies GABA and serotonin receptor ligands related to sedation and paradoxical excitation. Nature communications. 10:4078
Tuttle, A., Drerup, C.M., Marra, M., McGraw, H., Nechiporuk, A.V. (2019) Retrograde Ret signaling controls sensory pioneer axon outgrowth. eLIFE. 8:
Song, Y.C., Dohn, T.E., Rydeen, A.B., Nechiporuk, A.V., Waxman, J.S. (2019) HDAC1-mediated repression of the retinoic acid-responsive gene ripply3 promotes second heart field development. PLoS Genetics. 15:e1008165
Olson, H.M., Nechiporuk, A.V. (2018) Nothing to Be Sniffed At: Anosmin1 Tunes Fgf Diffusivity. Developmental Cell. 46:674-676
Olson, H.M., Nechiporuk, A.V. (2018) Using Zebrafish to Study Collective Cell Migration in Development and Disease. Frontiers in cell and developmental biology. 6:83
Ojeda Naharros, I., Gesemann, M., Mateos, J.M., Barmettler, G., Forbes, A., Ziegler, U., Neuhauss, S.C.F., Bachmann-Gagescu, R. (2017) Loss-of-function of the ciliopathy protein Cc2d2a disorganizes the vesicle fusion machinery at the periciliary membrane and indirectly affects Rab8-trafficking in zebrafish photoreceptors. PLoS Genetics. 13:e1007150
Herbert, A.L., Fu, M.M., Drerup, C.M., Gray, R.S., Harty, B.L., Ackerman, S.D., O'Reilly-Pol, T., Johnson, S.L., Nechiporuk, A.V., Barres, B.A., Monk, K.R. (2017) Dynein/dynactin is necessary for anterograde transport of Mbp mRNA in oligodendrocytes and for myelination in vivo.. Proceedings of the National Academy of Sciences of the United States of America. 114:E9153-E9162
Erickson, T., Morgan, C.P., Olt, J., Hardy, K., Busch-Nentwich, E.M., Maeda, R., Clemens-Grisham, R., Krey, J.F., Nechiporuk, A.V., Barr-Gillespie, P.G., Marcotti, W., Nicolson, T. (2017) Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt). eLIFE. 6
Drerup, C.M., Herbert, A.L., Monk, K.R., Nechiporuk, A.V. (2017) Regulation of mitochondria-dynactin interaction and mitochondrial retrograde transport in axons. eLIFE. 6
Bruni, G., Rennekamp, A.J., Velenich, A., McCarroll, M., Gendelev, L., Fertsch, E., Taylor, J., Lakhani, P., Lensen, D., Evron, T., Lorello, P.J., Huang, X.P., Kolczewski, S., Carey, G., Caldarone, B.J., Prinssen, E., Roth, B.L., Keiser, M.J., Peterson, R.T., Kokel, D. (2016) Zebrafish behavioral profiling identifies multitarget antipsychotic-like compounds. Nature Chemical Biology. 12(7):559-66
Drerup, C.M., Lusk, S., Nechiporuk, A. (2016) Kif1B Interacts with KBP to Promote Axon Elongation by Localizing a Microtubule Regulator to Growth Cones. The Journal of neuroscience : the official journal of the Society for Neuroscience. 36:7014-26
McCarroll, M.N., Gendelev, L., Keiser, M.J., Kokel, D. (2016) Leveraging large-scale behavioral profiling in zebrafish to explore neuroactive polypharmacology. ACS Chemical Biology. 11(4):842-9
Drerup, C.M., Nechiporuk, A.V. (2016) In vivo analysis of axonal transport in zebrafish. Methods in cell biology. 131:311-29
McGraw, H.F., Culbertson, M.D., Nechiporuk, A.V. (2014) Kremen1 restricts Dkk activity during posterior lateral line development in zebrafish. Development (Cambridge, England). 141(16):3212-21
Harding, M.J., McGraw, H.F., Nechiporuk, A. (2014) The roles and regulation of multicellular rosette structures during morphogenesis. Development (Cambridge, England). 141:2549-2558
McCarroll, M.N., and Nechiporuk, A.V. (2013) Fgf3 and Fgf10a work in concert to promote maturation of the epibranchial placodes in zebrafish. PLoS One. 8(12):e85087
Malmquist, S.J., Abramsson, A., McGraw, H.F., Linbo, T.H., Raible, D.W. (2013) Modulation of dorsal root ganglion development by ErbB signaling and the scaffold protein Sorbs3. Development (Cambridge, England). 140:3986-3996
Drerup, C.M., and Nechiporuk, A.V. (2013) JNK-Interacting Protein 3 Mediates the Retrograde Transport of Activated c-Jun N-Terminal Kinase and Lysosomes. PLoS Genetics. 9(2):e1003303
Obholzer, N., Swinburne, I.A., Schwab, E., Nechiporuk, A.V., Nicolson, T., and Megason, S.G. (2012) Rapid positional cloning of zebrafish mutations by linkage and homozygosity mapping using whole-genome sequencing. Development (Cambridge, England). 139(22):4280-4290
Harding, M.J., and Nechiporuk, A.V. (2012) Fgfr-Ras-MAPK signaling is required for apical constriction via apical positioning of Rho-associated kinase during mechanosensory organ formation. Development (Cambridge, England). 139(17):3130-3135
McCarroll, M.N., Lewis, Z.R., Culbertson, M.D., Martin, B.L., Kimelman, D., and Nechiporuk, A.V. (2012) Graded levels of Pax2a and Pax8 regulate cell differentiation during sensory placode formation. Development (Cambridge, England). 139(15):2740-2750
Dalgin, G., Ward, A.B., Hao le, T., Beattie, C.E., Nechiporuk, A., and Prince, V.E. (2011) Zebrafish mnx1 controls cell fate choice in the developing endocrine pancreas. Development (Cambridge, England). 138(21):4597-4608
McGraw, H.F., Drerup, C.M., Culbertson, M.D., Linbo, T., Raible, D.W., and Nechiporuk, A.V. (2011) Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium. Development (Cambridge, England). 138(18):3921-3930
Culbertson, M.D., Lewis, Z.R., and Nechiporuk, A.V. (2011) Chondrogenic and Gliogenic Subpopulations of Neural Crest Play Distinct Roles during the Assembly of Epibranchial Ganglia. PLoS One. 6(9):e24443
Mo, W., Chen, F., Nechiporuk, A., and Nicolson, T. (2010) Quantification of vestibular-induced eye movements in zebrafish larvae. BMC Neuroscience. 11:110
Kassen, S.C., Thummel, R., Campochiaro, L.A., Harding, M.J., Bennett, N.A., and Hyde, D.R. (2009) CNTF induces photoreceptor neuroprotection and Müller glial cell proliferation through two different signaling pathways in the adult zebrafish retina. Experimental Eye Research. 88(6):1051-1064
Nechiporuk, A., and Raible, D.W. (2008) FGF-Dependent Mechanosensory Organ Patterning in Zebrafish. Science (New York, N.Y.). 320(5884):1774-1777
Obholzer, N., Wolfson, S., Trapani, J.G., Mo, W., Nechiporuk, A., Busch-Nentwich, E., Seiler, C., Sidi, S., Söllner, C., Duncan, R.N., Boehland, A., and Nicolson, T. (2008) Vesicular glutamate transporter 3 is required for synaptic transmission in zebrafish hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(9):2110-2118
Nechiporuk, A., Linbo, T., Poss, K.D., and Raible, D.W. (2007) Specification of epibranchial placodes in zebrafish. Development (Cambridge, England). 134(3):611-623
Pyati, U.J., Cooper, M.S., Davidson, A.J., Nechiporuk, A., and Kimelman, D. (2006) Sustained Bmp signaling is essential for cloaca development in zebrafish. Development (Cambridge, England). 133(11):2275-2284
Nechiporuk, A., Linbo, T., and Raible, D.W. (2005) Endoderm-derived Fgf3 is necessary and sufficient for inducing neurogenesis in the epibranchial placodes in zebrafish. Development (Cambridge, England). 132(16):3717-3730
Poss, K.D., Nechiporuk, A., Stringer, K.F., Lee, C., and Keating, M.T. (2004) Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1. Genes & Development. 18(13):1527-1532
Nechiporuk, A., Poss, K.D., Johnson, S.L., and Keating, M.T. (2003) Positional cloning of a temperature-sensitive mutant emmental reveals a role for sly1 during cell proliferation in zebrafish fin regeneration. Developmental Biology. 258(2):291-306
Poss, K.D., Keating, M.T., and Nechiporuk, A. (2003) Tales of regeneration in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists. 226(2):202-210
Poss, K.D., Nechiporuk, A., Hillam, A.M., Johnson, S.L., and Keating, M.T. (2002) Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration. Development (Cambridge, England). 129(22):5141-5149
Nechiporuk, A. and Keating, M.T. (2002) A proliferation gradient between proximal and msxb-expressing distal blastema directs zebrafish fin regeneration. Development (Cambridge, England). 129(11):2607-2617
Nechiporuk, A. (2002) Molecular and cellular mechanisms of zebrafish fin regeneration. Ph.D. Thesis.
Poss, K.D., Shen, J., Nechiporuk, A., McMahon, G., Thisse, B., Thisse, C., and Keating, M.T. (2000) Roles for Fgf signaling during zebrafish fin regeneration. Developmental Biology. 222(2):347-358
Nechiporuk, A., Finney, J.E., Keating, M.T., and Johnson, S.L. (1999) Assessment of polymorphism in zebrafish mapping strains. Genome research. 9(12):1231-1238