ZFIN ID: ZDB-LAB-071012-1
Amack Lab
PI/Director: Amack, Jeffrey
Contact Person: Amack, Jeffrey
Email: AmackJ@upstate.edu
URL: http://amacklab.org
Address: Department of Cell and Developmental Biology State University of New York Upstate Medical University 309 Weiskotten Hall 750 E. Adams St. Syracuse, NY 13210
Country: United States
Phone: 315.464.8507
Fax: 315.464.8535
Line Designation: sny

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The focus of our lab is to understand the genetics and cell biology of organ morphogenesis during embryonic development. In particular, we are interested in how organs become asymmetric along the left-right (LR) body axis. In all vertebrates, the heart, brain and gastro-intestinal tract develop conserved LR differences during early steps of organogenesis. Perturbation of these LR asymmetries often leads to congenital disease. We are using zebrafish as a model vertebrate to identify genes and mechanisms that control asymmetric patterning and development.


Rathbun, L.I., Aljiboury, A.A., Bai, X., Hall, N.A., Manikas, J., Amack, J.D., Bembenek, J.N., Hehnly, H. (2020) PLK1- and PLK4-Mediated Asymmetric Mitotic Centrosome Size and Positioning in the Early Zebrafish Embryo. Current biology : CB. 30(22):4519-4527.e3
Colicino, E.G., Stevens, K., Curtis, E., Rathbun, L., Bates, M., Manikas, J., Amack, J., Freshour, J., Hehnly, H. (2019) Chromosome misalignment is associated with PLK1 activity at cenexin-positive mitotic centrosomes. Molecular biology of the cell. 30(13):1598-1609
Dasgupta, A., Jacob, A.E., Amack, J.D. (2018) Mosaic Labeling and 3-Dimensional Morphological Analysis of Single Cells in the Zebrafish Left-right Organizer. Bio-protocol. 8(22):
Erdemci-Tandogan, G., Clark, M.J., Amack, J.D., Manning, M.L. (2018) Tissue Flow Induces Cell Shape Changes During Organogenesis. Biophysical journal. 115(11):2259-2270
Saydmohammed, M., Yagi, H., Calderon, M., Clark, M.J., Feinstein, T., Sun, M., Stolz, D.B., Watkins, S.C., Amack, J.D., Lo, C.W., Tsang, M. (2018) Vertebrate myosin 1d regulates left-right organizer morphogenesis and laterality. Nature communications. 9:3381
Dasgupta, A., Merkel, M., Clark, M.J., Jacob, A.E., Dawson, J.E., Manning, M.L., Amack, J.D. (2018) Cell volume changes contribute to epithelial morphogenesis in zebrafish Kupffer's vesicle. eLIFE. 7
Jacob, A.E., Amack, J.D., Turner, C.E. (2017) Paxillin Genes and Actomyosin Contractility Regulate Myotome Morphogenesis in Zebrafish. Developmental Biology. 425(1):70-84
Dasgupta, A., Amack, J.D. (2016) Cilia in vertebrate left-right patterning. Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 371(1710)
Yu, M., Liu, Y., Li, J., Natale, B.N., Cao, S., Wang, D., Amack, J.D., Hu, H. (2016) Eyes shut homolog is required for maintaining the ciliary pocket and survival of photoreceptors in zebrafish. Biology Open. 5(11):1662-1673
Jacob, A.E., Turner, C.E., Amack, J.D. (2016) Evolution and Expression of Paxillin Genes in Teleost Fish. PLoS One. 11:e0165266
Ji, Y., Buel, S.M., Amack, J.D. (2016) Mutations in zebrafish pitx2 model congenital malformations in Axenfeld-Rieger syndrome but do not disrupt left-right placement of visceral organs. Developmental Biology. 416(1):69-81
Chang, C.T., Amack, J.D., Whipps, C.M. (2016) Zebrafish Embryo Disinfection with Povidone-Iodine: Evaluating an Alternative to Chlorine Bleach. Zebrafish. 13 Suppl 1:S96-S101
Gokey, J.J., Ji, Y., Tay, H.G., Litts, B., Amack, J.D. (2016) A Kupffer's vesicle size threshold for robust left-right patterning of the zebrafish embryo. Developmental dynamics : an official publication of the American Association of Anatomists. 245(1):22-33
Gokey, J.J., Dasgupta, A., Amack, J.D. (2015) The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish. Developmental Biology. 407(1):115-30
Fox, C., Manning, M.L., Amack, J.D. (2015) Quantitative description of fluid flows produced by left-right cilia in zebrafish. Methods in cell biology. 127:175-87
Lahvic, J.L., Ji, Y., Marin, P., Zuflacht, J.P., Springel, M.W., Wosen, J.E., Davis, L., Hutson, L.D., Amack, J.D., and Marvin, M.J. (2013) Small heat shock proteins are necessary for heart migration and laterality determination in zebrafish. Developmental Biology. 384(2):166-180
Neugebauer, J.M., Cadwallader, A.B., Amack, J.D., Bisgrove, B.W., and Yost, H.J. (2013) Differential roles for 3-OSTs in the regulation of cilia length and motility. Development (Cambridge, England). 140(18):3892-3902
Wang, W.J., Tay, H.G., Soni, R., Perumal, G.S., Goll, M.G., Macaluso, F.P., Asara, J.M., Amack, J.D., Tsou, M.F. (2013) CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.. Nature cell biology. 15(6):591-601
Tay, H.G., Schulze, S.K., Compagnon, J., Foley, F.C., Heisenberg, C.P., Yost, H.J., Abdelilah-Seyfried, S., and Amack, J.D. (2013) Lethal giant larvae 2 regulates development of the ciliated organ Kupffer's vesicle. Development (Cambridge, England). 140(7):1550-1559
Wang, G., Yost, H.J., and Amack, J.D. (2013) Analysis of Gene Function and Visualization of Cilia-Generated Fluid Flow in Kupffer's Vesicle. Journal of visualized experiments : JoVE. (73)
Wang, G., Manning, M.L., and Amack, J.D. (2012) Regional Cell Shape Changes Control Form and Function of Kupffer's Vesicle in the Zebrafish Embryo. Developmental Biology. 370(1):52-62
French, V.M., van de Laar, I.M., Wessels, M.W., Rohe, C., Roos-Hesselink, J.W., Wang, G., Frohn-Mulder, I.M., Severijnen, L.A., de Graaf, B.M., Schot, R., Breedveld, G., Mientjes, E., van Tienhoven, M., Jadot, E., Jiang, Z., Verkerk, A., Swagemakers, S., Venselaar, H., Rahimi, Z., Najmabadi, H., Meijers-Heijboer, H., de Graaff, E., Helbing, W.A., Willemsen, R., Devriendt, K., Belmont, J.W., Oostra, B.A., Amack, J.D., and Bertoli-Avella, A.M. (2012) NPHP4 Variants Are Associated With Pleiotropic Heart Malformations. Circulation research. 110(12):1564-1574
Cast, A.E., Gao, C., Amack, J.D., and Ware, S.M. (2012) An essential and highly conserved role for Zic3 in left-right patterning, gastrulation and convergent extension morphogenesis. Developmental Biology. 364(1):22-31
Yu, J.A., Foley, F.C., Amack, J.D., and Turner, C.E. (2011) The Cell Adhesion-associated Protein Git2 Regulates Morphogenetic Movements during Zebrafish Embryonic Development. Developmental Biology. 349(2):225-237
Wang, G., Cadwallader, A.B., Jang, D.S., Tsang, M., Yost, H.J., and Amack, J.D. (2011) The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer's vesicle in zebrafish. Development (Cambridge, England). 138(1):45-54
Gao, C., Wang, G., Amack, J.D., and Mitchell, D.R. (2010) Oda16/Wdr69 is essential for axonemal dynein assembly and ciliary motility during zebrafish embryogenesis. Developmental dynamics : an official publication of the American Association of Anatomists. 239(8):2190-2197
Neugebauer, J.M., Amack, J.D., Peterson, A.G., Bisgrove, B.W., and Yost, H.J. (2009) FGF signalling during embryo development regulates cilia length in diverse epithelia. Nature. 458(7238):651-654
Amack, J.D., Wang, X., and Yost, H.J. (2007) Two T-box genes play independent and cooperative roles to regulate morphogenesis of ciliated Kupffer's vesicle in zebrafish. Developmental Biology. 310(2):196-210
Tsai, I.C., Amack, J.D., Gao, Z.H., Band, V., Yost, H.J., and Virshup, D.M. (2007) A Wnt-CKIε-Rap1 Pathway Regulates Gastrulation by Modulating SIPA1L1, a Rap GTPase Activating Protein. Developmental Cell. 12(3):335-347
Essner, J.J., Amack, J.D., Nyholm, M.K., Harris, E.B., and Yost, H.J. (2005) Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut. Development (Cambridge, England). 132(6):1247-1260
Amack, J.D., and Yost, H.J. (2004) The T box transcription factor no tail in ciliated cells controls zebrafish left-right asymmetry. Current biology : CB. 14(8):685-690