ZFIN ID: ZDB-LAB-050531-2
Mione Lab
PI/Director: Mione, Marina
Contact Person: Mione, Marina
Email: mariacaterina.mione@unitn.it
URL: http://www.cibio.unitn.it/465/laboratory-of-experimental-cancer-biology
Address: Centre for Integrative Biology (CIBIO) University of Trento Via Sommarive 9, 38123 Trento - Italy
Country: Italy
Phone: +39 0461 28 3312
Fax: +39 0461 28 3937
Line Designation: utn

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The use of the zebrafish to study cancer is an emerging field, and my laboratory has developed systems that allow to visualize oncogenic transformation of cells in different organs and tissues of transparent zebrafish larvae through the use of GFP-tagged oncogenes. Briefly, we use genetic systems for conditional and mosaic expression of oncogenes recapitulating the occurrence of somatic mutations in sporadic cancer. These systems also allow us to precisely target transformed cells with different genes to study the role of their products in transformation. Using this approach, we have studied the biology of cancer initiating cells in their native environment in models of melanoma (Santoriello et al., 2010) and glioblastoma (Mione et al., in preparation), and identified cellular and extracellular factors that allow them to thrive and establish cancer. These systems are also ideal to visualize and manipulate the early immune responses to cancer cells in a live vertebrate organism.

Anelli, Viviana Post-Doc Idilli, Aurora Post-Doc Precazzini, Francesca Graduate Student

Rosello, M., Vougny, J., Czarny, F., Mione, M.C., Concordet, J.P., Albadri, S., Del Bene, F. (2021) Precise base editing for the in vivo study of developmental signaling and human pathologies in zebrafish. eLIFE. 10:
Idilli, A.I., Pazzi, C., Dal Pozzolo, F., Roccuzzo, M., Mione, M.C. (2020) Rad21 Haploinsufficiency Prevents ALT-Associated Phenotypes in Zebrafish Brain Tumors. Genes. 11(12):
Lefkopoulos, S., Polyzou, A., Derecka, M., Bergo, V., Clapes, T., Cauchy, P., Jerez-Longres, C., Onishi-Seebacher, M., Yin, N., Martagon-Calderón, N.A., Potts, K.S., Klaeylé, L., Liu, F., Bowman, T.V., Jenuwein, T., Mione, M.C., Trompouki, E. (2020) Repetitive Elements Trigger RIG-I-like Receptor Signaling that Regulates the Emergence of Hematopoietic Stem and Progenitor Cells. Immunity. 53:934-951.e9
Idilli, A.I., Pagani, F., Kerschbamer, E., Berardinelli, F., Bernabé, M., Cayuela, M.L., Piazza, S., Poliani, P.L., Cusanelli, E., Mione, M.C. (2020) Changes in the Expression of Pre-Replicative Complex Genes in hTERT and ALT Pediatric Brain Tumors. Cancers. 12(4)
Idilli, A.I., Cusanelli, E., Pagani, F., Berardinelli, F., Bernabé, M., Cayuela, M.L., Poliani, P.L., Mione, M.C. (2020) Expression of tert Prevents ALT in Zebrafish Brain Tumors. Frontiers in cell and developmental biology. 8:65
Misselbeck, K., Parolo, S., Lorenzini, F., Savoca, V., Leonardelli, L., Bora, P., Morine, M.J., Mione, M.C., Domenici, E., Priami, C. (2019) A network-based approach to identify deregulated pathways and drug effects in metabolic syndrome. Nature communications. 10:5215
Gómez-Abenza, E., Ibáñez-Molero, S., García-Moreno, D., Fuentes, I., Zon, L.I., Mione, M.C., Cayuela, M.L., Gabellini, C., Mulero, V. (2019) Zebrafish modeling reveals that SPINT1 regulates the aggressiveness of skin cutaneous melanoma and its crosstalk with tumor immune microenvironment. Journal of experimental & clinical cancer research : CR. 38:405
Precazzini, F., Pancher, M., Gatto, P., Tushe, A., Adami, V., Anelli, V., Mione, M.C. (2019) Automated in vivo screen in zebrafish identifies clotrimazole as targeting a metabolic vulnerability in a melanoma model. Developmental Biology. 457(2):215-225
Dickmeis, T., Feng, Y., Mione, M.C., Ninov, N., Santoro, M., Spaink, H.P., Gut, P. (2019) Nano-Sampling and Reporter Tools to Study Metabolic Regulation in Zebrafish. Frontiers in cell and developmental biology. 7:15
Salama, M., Benitez-Riquelme, D., Elabd, S., Munoz, L., Zhang, P., Glanemann, M., Mione, M.C., Goldin, R., Soussi, T., Davidson, G., Blattner, C. (2019) Fam83F induces p53 stabilisation and promotes its activity. Cell death and differentiation. 26(10):2125-2138
Cayuela, M.L., Claes, K.B.M., Ferreira, M.G., Henriques, C.M., van Eeden, F., Varga, M., Vierstraete, J., Mione, M.C. (2019) The Zebrafish as an Emerging Model to Study DNA Damage in Aging, Cancer and Other Diseases. Frontiers in cell and developmental biology. 6:178
Zizioli, D., Mione, M., Varinelli, M., Malagola, M., Bernardi, S., Alghisi, E., Borsani, G., Finazzi, D., Monti, E., Presta, M., Russo, D. (2018) Zebrafish disease models in hematology: Highlights on biological and translational impact. Biochimica et biophysica acta. Molecular basis of disease. 1865(3):620-633
Anelli, V., Ordas, A., Kneitz, S., Sagredo, L.M., Gourain, V., Schartl, M., Meijer, A.H., Mione, M. (2018) Ras-Induced miR-146a and 193a Target Jmjd6 to Regulate Melanoma Progression. Frontiers in genetics. 9:675
Piragyte, I., Clapes, T., Polyzou, A., Klein Geltink, R.I., Lefkopoulos, S., Yin, N., Cauchy, P., Curtis, J.D., Klaeylé, L., Langa, X., Beckmann, C.C.A., Wlodarski, M.W., Müller, P., Van Essen, D., Rambold, A., Kapp, F.G., Mione, M., Buescher, J.M., Pearce, E.L., Polyzos, A., Trompouki, E. (2018) A metabolic interplay coordinated by HLX regulates myeloid differentiation and AML through partly overlapping pathways. Nature communications. 9:3090
Schorch, B., Heni, H., Zahaf, N.I., Brummer, T., Mione, M., Schmidt, G., Papatheodorou, P., Aktories, K. (2018) Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL.. Oncotarget. 9:16489-16500
Chia, K., Mazzolini, J., Mione, M., Sieger, D. (2018) Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain. eLIFE. 7
Idilli, A.I., Precazzini, F., Mione, M.C., Anelli, V. (2017) Zebrafish in Translational Cancer Research: Insight into Leukemia, Melanoma, Glioma and Endocrine Tumor Biology. Genes. 8(9)
Ibarra, M.S., Borini Etichetti, C., Di Benedetto, C., Rosano, G.L., Margarit, E., Del Sal, G., Mione, M., Girardini, J. (2017) Dynamic regulation of Pin1 expression and function during zebrafish development. PLoS One. 12:e0175939
Anelli, V., Villefranc, J.A., Chhangawala, S., Martinez-McFaline, R., Riva, E., Nguyen, A., Verma, A., Bareja, R., Chen, Z., Scognamiglio, T., Elemento, O., Houvras, Y. (2017) Oncogenic BRAF disrupts thyroid morphogenesis and function via Twist expression. eLIFE. 6
Mayrhofer, M., Gourain, V., Reischl, M., Affaticati, P., Jenett, A., Joly, J.S., Benelli, M., Demichelis, F., Poliani, P.L., Sieger, D., Mione, M. (2017) A novel brain tumour model in zebrafish reveals the role of YAP activation in MAPK/PI3K induced malignant growth. Disease models & mechanisms. 10(1):15-28
Schutera, M., Dickmeis, T., Mione, M., Peravali, R., Marcato, D., Reischl, M., Mikut, R., Pylatiuk, C. (2016) Automated Phenotype Pattern Recognition of Zebrafish for High-throughput Screening. Bioengineered. 7(4):261-5
Feng, Y., Santoriello, C., Mione, M., Hurlstone, A., Martin, P. (2016) Correction: Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology. 14:e1002377
Mayrhofer, M., Mione, M. (2016) The Toolbox for Conditional Zebrafish Cancer Models. Advances in experimental medicine and biology. 916:21-59
Arbizzani, F., Mayrhofer, M., Mione, M. (2015) Novel Transgenic Lines to Fluorescently Label Clathrin and Caveolin Endosomes in Live Zebrafish. Zebrafish. 12(2):202-3
Ogrunc, M., Di Micco, R., Liontos, M., Bombardelli, L., Mione, M., Fumagalli, M., Gorgoulis, V.G., d'Adda di Fagagna, F. (2014) Oncogene-induced reactive oxygen species fuel hyperproliferation and DNA damage response activation. Cell death and differentiation. 21:998-1012
Vacaru, A.M., Unlu, G., Spitzner, M., Mione, M., Knapik, E.W., and Sadler, K.C. (2014) In vivo cell biology in zebrafish - providing insights into vertebrate development and disease. Journal of Cell Science. 127(Pt 3):485-495
Alghisi, E., Distel, M., Malagola, M., Anelli, V., Santoriello, C., Herwig, L., Krudewig, A., Henkel, C.V., Russo, D., and Mione, M.C. (2013) Targeting oncogene expression to endothelial cells induces proliferation of the myelo-erythroid lineage by repressing the notch pathway. Leukemia. 24(6):943-53
Colombo, A., Palma, K., Armijo, L., Mione, M., Signore, I.A., Morales, C., Guerrero, N., Meynard, M.M., Pérez, R., Suazo, J., Marcelain, K., Briones, L., Härtel, S., Wilson, S.W., and Concha, M.L. (2013) Daam1a mediates asymmetric habenular morphogenesis by regulating dendritic and axonal outgrowth. Development (Cambridge, England). 140(19):3997-4007
Tran, L.D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M., and Sampath, K. (2012) Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. Development (Cambridge, England). 139(19):3644-3652
Francia, S., Michelini, F., Saxena, A., Tang, D., de Hoon, M., Anelli, V., Mione, M., Carninci, P., and di Fagagna, F.D. (2012) Site-specific DICER and DROSHA RNA products control the DNA-damage response. Nature. 488(7410):231-235
Mione, M., and Zon, L.I. (2012) Cancer and inflammation: an aspirin a day keeps the cancer at bay. Current biology : CB. 22(13):R522-525
York, A.G., Parekh, S.H., Nogare, D.D., Fischer, R.S., Temprine, K., Mione, M., Chitnis, A.B., Combs, C.A., and Shroff, H. (2012) Resolution doubling in live, multicellular organisms via multifocal structured illumination microscopy. Nature Methods. 9(7):749-754
Santoriello, C., Anelli, V., Alghisi, E., and Mione, M. (2012) Highly penetrant melanoma in a zebrafish model is independent of ErbB3b signaling. Pigment cell & melanoma research. 25(2):287-289
Du, S., Draper, B.W., Mione, M., Moens, C.B., and Bruce, A.E. (2012) Differential regulation of epiboly initiation and progression by zebrafish Eomesodermin A. Developmental Biology. 362(1):11-23
Bassi, A., Fieramonti, L., D'Andrea, C., Mione, M., and Valentini, G. (2011) In vivo label-free three-dimensional imaging of zebrafish vasculature with optical projection tomography. Journal of Biomedical Optics. 16(10):100502
Guarani, V., Deflorian, G., Franco, C.A., Krüger, M., Phng, L.K., Bentley, K., Toussaint, L., Dequiedt, F., Mostoslavsky, R., Schmidt, M.H., Zimmermann, B., Brandes, R.P., Mione, M., Westphal, C.H., Braun, T., Zeiher, A.M., Gerhardt, H., Dimmeler, S., and Potente, M. (2011) Acetylation-dependent regulation of endothelial Notch signalling by the SIRT1 deacetylase. Nature. 473(7346):234-238
Richardson, J., Zeng, Z., Ceol, C., Mione, M., Jackson, I.J., and Patton, E.E. (2011) A zebrafish model for nevus regeneration. Pigment cell & melanoma research. 24(2):378-381
Bernardi, E., Deflorian, G., Pezzinenti, F., Diaz, V.M., Mione, M., and Blasi, F. (2010) Characterization of the Regulatory Region of the Zebrafish Prep1.1 Gene: Analogies to the Promoter of the Human PREP1. PLoS One. 5(12):e15047
Feng, Y., Santoriello, C., Mione, M., Hurlstone, A., and Martin, P. (2010) Live Imaging of Innate Immune Cell Sensing of Transformed Cells in Zebrafish Larvae: Parallels between Tumor Initiation and Wound Inflammation. PLoS Biology. 8(12):e1000562
Santoriello, C., Gennaro, E., Anelli, V., Distel, M., Kelly, A., Köster, R.W., Hurlstone, A., and Mione, M. (2010) Kita Driven Expression of Oncogenic HRAS Leads to Early Onset and Highly Penetrant Melanoma in Zebrafish. PLoS One. 5(12):e15170
Mione, M.C., and Trede, N.S. (2010) The zebrafish as a model for cancer. Disease models & mechanisms. 3(9-10):517-523
Anelli, V., Santoriello, C., Distel, M., Köster, R.W., Ciccarelli, F.D., and Mione, M. (2009) Global repression of cancer gene expression in a zebrafish model of melanoma is linked to epigenetic regulation. Zebrafish. 6(4):417-424
Mione, M., Meijer, A.H., Snaar-Jagalska, B.E., Spaink, H.P., and Trede, N.S. (2009) Disease modeling in zebrafish: cancer and immune responses-a report on a workshop held in Spoleto, Italy, July 20-22, 2009. Zebrafish. 6(4):445-451
Deflorian, G., Cinquanta, M., Beretta, C., Venuto, A., Santoriello, C., Baldessari, D., Pezzimenti, F., Aliprandi, M., Mione, M., and de Marco, A. (2009) Monoclonal antibodies isolated by large-scale screening are suitable for labeling adult zebrafish (Danio rerio) tissues and cell structures. Journal of immunological methods. 346(1-2):9-17
Montero-Balaguer, M., Swirsding, K., Orsenigo, F., Cotelli, F., Mione, M., and Dejana, E. (2009) Stable vascular connections and remodeling require full expression of VE-cadherin in zebrafish embryos. PLoS One. 4(6):e5772
Stone, O.A., Richer, C., Emanueli, C., van Weel, V., Quax, P.H., Katare, R., Kraenkel, N., Campagnolo, P., Barcelos, L.S., Siragusa, M., Sala-Newby, G.B., Baldessari, D., Mione, M., Vincent, M.P., Benest, A.V., Al Haj Zen, A., Gonzalez, J., Bates, D.O., Alhenc-Gelas, F., and Madeddu, P. (2009) Critical Role of Tissue Kallikrein in Vessel Formation and Maturation: Implications for Therapeutic Revascularization. Arterioscler. Thromb. Vasc. Biol.. 29(5):657-664
Meani, N., Pezzimenti, F., Deflorian, G., Mione, M., and Alcalay, M. (2009) The tumor suppressor PRDM5 regulates Wnt signaling at early stages of zebrafish development. PLoS One. 4(1):e4273
Santoriello, C., Deflorian, G., Pezzimenti, F., Kawakami, K., Lanfrancone, L., d'Adda di Fagagna, F., and Mione, M. (2009) Expression of H-RASV12 in a zebrafish model of Costello syndrome causes cellular senescence in adult proliferating cells. Disease models & mechanisms. 2(1-2):56-67
Palamidessi, A., Frittoli, E., Garré, M., Faretta, M., Mione, M., Testa, I., Diaspro, A., Lanzetti, L., Scita, G., and Di Fiore, P.P. (2008) Endocytic trafficking of rac is required for the spatial restriction of signaling in cell migration. Cell. 134(1):135-147
Pocock, R., Mione, M., Hussain, S., Maxwell, S., Pontecorvi, M., Aslam, S., Gerrelli, D., Sowden, J.C., and Woollard, A. (2008) Neuronal function of Tbx20 conserved from nematodes to vertebrates. Developmental Biology. 317(2):671-685
Baldessari, D., and Mione, M. (2008) How to create the vascular tree? (Latest) help from the zebrafish. Pharmacology & Therapeutics. 118(2):206-230
Aleström, P., Begemann, G., Carvan, M.J. 3rd, Cheng, K.C., Crosier, K., Crosier, P., Ekker, S., Huttenlocher, A., Kawakami, K., Kelly, G., Korzh, V., Lieschke, G., Mione, M., Neely, M.N., Neuhauss, S., and Trede, N.S. (2008) Views on four key questions about zebrafish research. Zebrafish. 5(1):9-24
Mione, M., Baldessari, D., Deflorian, G., Nappo, G., and Santoriello, C. (2008) How neuronal migration contributes to the morphogenesis of the CNS: insights from the zebrafish. Developmental neuroscience. 30(1-3):65-81
Potente, M., Ghaeni, L., Baldessari, D., Mostoslavsky, R., Rossig, L., Dequiedt, F., Haendeler, J., Mione, M., Dejana, E., Alt, F.W., Zeiher, A.M., and Dimmeler, S. (2007) SIRT1 controls endothelial angiogenic functions during vascular growth. Genes & Development. 21(20):2644-2658
Costagli, A., Felice, B., Guffanti, A., Wilson, S.W., and Mione, M. (2006) Identification of alternatively spliced dab1 isoforms in zebrafish. Development genes and evolution. 216(6):291-299
Foucher, I., Mione, M., Simeone, A., Acampora, D., Bally-Cuif, L., and Houart, C. (2006) Differentiation of cerebellar cell identities in absence of Fgf signalling in zebrafish Otx morphants. Development (Cambridge, England). 133(10):1891-1900
Mione, M., Lele, Z., Kwong, C.T., Concha, M.L., and Clarke, J.D. (2006) Expression of pcp4a in subpopulations of CNS neurons in zebrafish. The Journal of comparative neurology. 495(6):769-787
Colombo, A., Reig, G., Mione, M., and Concha, M.L. (2006) Zebrafish BarH-like genes define discrete neural domains in the early embryo. Gene expression patterns : GEP. 6(4):347-352
Jeong, J.Y., Einhorn, Z., Mercurio, S., Lee, S., Lau, B., Mione, M., Wilson, S.W., and Guo, S. (2006) Neurogenin1 is a determinant of zebrafish basal forebrain dopaminergic neurons and is regulated by the conserved zinc finger protein Tof/Fezl. Proceedings of the National Academy of Sciences of the United States of America. 103(13):5143-5148
Li, J., Mack, J.A., Souren, M., Yaksi, E., Higashijima, S., Mione, M., Fetcho, J.R., and Friedrich, R.W. (2005) Early development of functional spatial maps in the zebrafish olfactory bulb. The Journal of neuroscience : the official journal of the Society for Neuroscience. 25(24):5784-95
Costagli, A., Kapsimali, M., Wilson, S.W., and Mione, M. (2002) Conserved and divergent patterns of Reelin expression in the zebrafish central nervous system. The Journal of comparative neurology. 450(1):73-93
Wendl, T., Lun, K., Mione, M., Favor, J., Brand, M., Wilson, S.W., and Rohr, K.B. (2002) pax2.1 is required for the development of thyroid follicles in zebrafish. Development (Cambridge, England). 129(15):3751-3760
Mione, M., Shanmugalingam, S., Kimelman, D., and Griffin, K. (2001) Overlapping expression of zebrafish T-brain-1 and eomesodermin during forebrain development. Mechanisms of Development. 100(1):93-97