Figure 5.
- ID
- ZDB-IMAGE-260902-6
- Publication
- Gan et al., 2026 - COMMD9-regulated endothelial cell abnormality-induced hypercoagulability is associated with Budd-Chiari syndrome
- All Figures
- Figures for Gan et al., 2026
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Figure 5.
COMMD9 knockdown contributes to endothelial cell abnormalities via the PI3K-Akt signaling pathway. (A) Heatmap of differentially expressed genes identified by RNA-seq in HHSECs transfected with negative-control siRNA (siNC) or siRNA targeting COMMD9 (log2FC > 1.5 or < −1.5 and P < 0.05; N = 3 biological replicates). (B) Volcano plot showing upregulated differentially expressed genes in red and downregulated genes in blue. (C) Top 10 GO terms and 4 KEGG signaling pathways were significantly enriched among upregulated genes. (D) qPCR analysis confirming the upregulation of PI3K-Akt signaling pathway-related genes following COMMD9 knockdown in HHSECs. (E) Cell adhesion assay of HHSECs with or without VO-Ohpic upon COMMD9 knockdown. (F) Migration assay of HHSECs with or without VO-Ohpic upon COMMD9 knockdown. (G) Tube formation assays of HHSECs with or without VO-Ohpic. Scale bar, 100 μm. N = 3 biological replicates. The results are shown as mean ± SD, ****P < 0.0001; ***P < 0.001; **P < 0.01; *P < 0.05; n.s., not significant.