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Fig. 4

ID: ZDB-IMAGE-260716-56
Publication: Wang et al., 2026 - Checkpoint kinase 2 coordinates autophagy activation and Aurora kinase A degradation to regulate primary cilia for cell invasion
All Figures: Figures for Wang et al., 2026

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Fig. 4

CHEK2 promotes trophoblast cell invasion upon serum deprivation. A-B Serum starvation promotes trophoblast HTR-8 cell migration and invasion. Left: visualization of cells that migrated to the lower chamber. Right: Quantitative results of (A) migrated or (B) invaded cells in full-medium (FM) or serum-starved medium (Starv.). C-E Western blotting showing that serum starvation reduced (C) E-cadherin (E-cad, epithelial marker), but promoted (D) N-cadherin (N-cad, mesenchymal marker) and (E) vimentin (VIM, mesenchymal marker). Ku70 and actin are gel-loading controls. F-G Depletion of CHEK2 by siCHEK2 inhibits trophoblast HTR-8 cell (F) migration and (G) invasion. H-L Depletion of CEP164 inhibited primary cilia, cell migration, and invasion. H-I Efficient depletion of CEP164 by siRNA. H Extracts of cells transfected with siCEP164 were analyzed by Western blotting with antibodies against CEP164 and Ku70. I Quantitative results for the relative intensity of CEP164 in H. Reduced numbers of (J) ciliated, (K) migrated, or (L) invaded HTR-8 cells in the presence of siCEP164 under serum starvation

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