Figure 3.
- ID
- ZDB-IMAGE-260509-17
- Publication
- Gillotay et al., 2026 - The role of Nrf2 in thyroid maturation and hormone synthesis in vertebrate models
- All Figures
- Figures for Gillotay et al., 2026
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Figure 3.
Thyroid-specific rescue of nrf2a function is sufficient to recover physiological T4 production.
(A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P) Immunofluorescence on 6 dpf Tg(tg:nlsEGFP; tg:nrf2aT2A_mKO2) zebrafish embryos targeting GFP and thyroxine (T4-Cy3, red) reveals that the presence of the tg:nrf2aT2A_mKO2 construct is sufficient to rescue the thyroid hormone production defect in nrf2a homozygous mutants compared with their WT and heterozygous mutant siblings (A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P). (A, B, C, D) show whole head pictures of representative embryos of each genotype (ventral view, anterior to the left). (E, F, G, H, I, J, K, L, M, N, O, P) Enlarged views of the thyroid of the corresponding embryos demonstrate thyroid follicular organization in all embryos (E, F, G, H) and reveal that the absence of T4 staining observed in nrf2a homozygous mutant embryos (second column to the left) is rescued in nrf2a homozygous mutant embryos expressing de tg:nrf2aT2A_mKO2 transgene (last column to the left). Scale bars: 50 μm. (Q) Quantification of colloidal thyroxine immunofluorescence signal in individual WT (n = 25), nrf2a homozygous mutant embryos (n = 32), WT nrf2aT2A_mKO2 (n = 25), and nrf2a homozygous mutant nrf2aT2A_mKO2 embryos (n = 21) confirmed the rescue of the T4 production in the nrf2a homozygous mutant nrf2aT2A_mKO2 embryo group compared with their siblings from other genotypes. Results are shown as the mean ± SD. The asterisk denotes significant differences between groups of embryos (****P < 0.0001, one-way ANOVA multiple comparison test). (R) Quantification of thyroid follicular cell (TFC) number in individual WT (n = 15) and homozygous nrf2a mutant embryos (n = 24) and WT (n = 15) and homozygous nrf2a mutant (n = 11) nrf2aT2A_mKO2 embryos shows that the nrf2aT2A_mKO2 transgene rescued the thyroid cell number in homozygous nrf2a mutant embryos. Results are shown as the mean ± SD. The asterisk denotes significant differences between groups of embryos (**P < 0.01, Kruskal–Wallis multiple comparison test).