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Figure S2.

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ZDB-IMAGE-260509-14
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Gillotay et al., 2026 - The role of Nrf2 in thyroid maturation and hormone synthesis in vertebrate models
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Figure S2. Loss-of-function of nrf2a does not impair thyroid follicle polarization.
(A, B, C, D, E, F) Immunofluorescence on 6 dpf Tg(tg:nlsEGFP) zebrafish embryos using antibodies targeting EGFP and ZO-1 (Cy3, red) reveals normal follicular polarization in nrf2a homozygous mutant compared with their WT siblings (A, B, C, D, E, F). The upper row shows GFP staining only, the middle row shows ZO-1 staining only, and the last row shows a merge of both staining. White arrows show representative follicles in both WT and homozygous mutants. Scale bars: 10 μm. Ventral view, anterior to the left.

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