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Chen et al., 2026 - Insulin-like growth factor signaling regulates zebrafish lymphatic-vessel development

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Fig. 3 pappa2 is downregulated in kat6ab719 chondrocytes (A) Diagram illustrating the workflow for the RNA-seq experiment. (B) Gel electrophoresis of RT-PCR products demonstrating the purity of FACS-isolated chondrocytes from col2a1a:mCherry; sox10:EGFP larvae. mCherry/EGFP double-positive chondrocytes express mCherry, col2a1a and the housekeeping gene ef1α (eef1a1l1) but do not express the neutrophil marker lyz. In comparison, mCherry/EGFP double-negative non-chondrocytes express ef1α and lyz. (C) Volcano plot showing the DEGs and those DEGs that encode secreted proteins. Red and blue dots represent the significantly downregulated and upregulated genes (p < 0.01). The green dot indicates the candidate gene pappa2. (D) RT-qPCR analysis of hoxb2a and pappa2 expression confirming the downregulation of pappa2 in kat6ab719 chondrocytes. Bar graph data represent mean ± SD (n = 3 experiments).

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