ZFIN Image: Han et al., 2026, FIGURE 3

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FIGURE 3

Validation of add3a upregulation via mRNA overexpression and knockdown using MOs. (A) RT-PCR confirmed successful overexpression of add3a, demonstrated by an increased level of add3a mRNA. (B) The target sites of add3a-SBMO and add3a-TBMO are shown. (C) RT-PCR and gel electrophoresis verified the effectiveness of add3a-SBMO, indicated by a decrease in band intensity. (D) The diminished fluorescence of PEGFP-N1 following co-injection with add3a-TBMO confirmed the effectiveness of add3a-TBMO (n = 20).

Acknowledgments

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