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Fig. 2 Ube3a mutants exhibit altered social preference but not nonsocial preference in the styrofoam environment. a Scheme illustrating the experimental setup for social preference using conspecifics; created with BioRender. b Cumulative time spent in the ROI (shoal or empty) for WT and mutants (N = 10 for WT, N = 12 for mutant, Wilcoxon matched-pairs signed rank test). c Social preference-related parameters measured for the WT and mutants (N = 10 for WT, N = 12 for mutant; unpaired t test). d Scheme illustrating the experimental setup for nonsocial preference using glass marbles; created with BioRender. e Cumulative time spent in the ROI (object or empty) for WT and mutants (N = 15 for WT, N = 14 for mutant, Wilcoxon matched-pairs signed rank test). f Nonsocial preference-related parameters measured for the WT and mutants (N = 15 for WT, N = 14 for mutant; unpaired t test). The data are presented as the mean ± SEM. ns: not significant, *P < 0.05, **P < 0.01 and ***P < 0.001.