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Fig. 2 Mitosis of RPCs in the CMZ of 5 dpf retinas. (A) Immunostaining with phospho-histone H3 (PH3) reveals mitotic cells in central retinal cryosections from wild-type (Ctrl) and mutant (smarce1-/-) samples at 5 dpf. DAPI staining labels nuclei. A reduced number of PH3-positive cells is observed in smarce1-/- retinas compared to Ctrl. Scale bar: 50 μm. (B) Quantification of PH3 positive cells in WT and smarce1-/- retinas. The upper graph shows the average number of PH3 cells per section (7–10 sections); each dot represents one retina. For both control and smarce1-/- mutants, n = 10. The lower graph shows the total number of PH3-positive cells per retina. A significant reduction in PH3-positive cells was observed in smarce-/- samples compared to Ctrl (cells per section: t18 = 4.14, ***p = 0.0006; in total cells U = 9 **p = 0.002).