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Fig. 4 (A) Representative immunofluorescence images of 5-day-old wild type and CDH23-knockdown zebrafish retina. Sections were stained with MPDZ (green) and DAPI (blue). Scale bar: 10 µm. (B) Effects of CDH23 knockdown on OS length and ONL cell density in zebrafish retinas. Bar graphs present mean OS length (μm) and ONL cell counts (cells/10 µm ONL) for wild type and CDH23-knockdown groups. Sample sizes: n=5 for OS length and n=3 for ONL cell density. Data are expressed as mean±SEM; *p<0.05, **p<0.01 (independent-samples t-test) (Representative images shown). DAPI, 4′,6-Diamidino-2-Phenylindole; GCL, ganglion cell layer; INL, inner nuclear layer; IS, inner segment; ONL, outer nuclear layer; OS, outer segment; RPE, retinal pigment epithelium.