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Fig. 5 A comparison of detection of EC proliferation by PCNA immunolabelling, EdU cell labelling and time-lapse imaging of fluorescently labelled EC nuclei. Endothelial cell proliferation was studied at 30 hpf in PCNA treated embryos, 30 to 31 hpf in EdU treated embryos, and 29–45 hpf in time-lapse imaged embryos. Time-lapse imaging detected EC proliferation more effectively than other methods. Statistics were derived from analysis by ordinary one-way ANOVA.
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