Fig. 5

Fig. 5 Cmpk2 deficiency impairs neutrophil phagocytosis of bacteria in a STING-dependent manner A-B Representative images A and quantification B of flow cytometry of WT/KO neutrophil phagocytosis to P. aeruginosa. C Experimental strategy for bacterial infection of zebrafish embryos. WT and KO zebrafish embryos were infected with 1 × 106 CFU, followed by analyses at 36 h post administration. D The bacterial clone count of zebrafish embryos infected with S. aureus or P. aeruginosa. E-F the survival analysis of zebrafish embryos infected with P. aeruginosa E or S. aureus F (n = 23 per group). G Representative immunoblots of cGAS and STING protein expression in neutrophils infected with P. aeruginosa for 1 h. H Quantification of flow cytometry of WT/KO neutrophil phagocytosis to P. aeruginosa. I-J The bacterial clone count of zebrafish embryos infected with P. aeruginosa I or S. aureus J for 24 h. K-L The survival analysis of zebrafish embryos infected with P. aeruginosa K or S. aureus L (n = 23 per group). Each data point represents an individual mouse B, H or zebrafish embryo D, I-J. ns, not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (by two-tailed unpaired t-test in B and by one-way ANOVA followed by Tukey’s post hoc test in D, H-J