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Fig. 3 - Supplemental 1 Examination on the functional genes and pathways associated with the inflammatory bowel disease (IBD)-like phenotype in ctla-4-/- zebrafish. (A) Top five KEGG enrichment bar plot of up-regulated genes in ctla-4-/- zebrafish intestines versus wild-type (WT) zebfish intestines. (B) Top 10 KEGG enrichment bar plot of down-regulated genes in ctla-4-/- zebrafish intestines versus WT zebrafish intestines. (C, D) Changes in the expression of genes associated with lymphocyte chemotaxis, positive regulation of ERK1/ERK2 cascades, Calcium and MAPK signaling pathways in the ctla-4-/- zebrafish intestines analyzed by using a collection of pre-defined gene sets retrieved from GO (C) and KEGG (D) database. The p-value, false discovery rates (FDR), and normalized enrichment score (NES) are shown above each pathway graph.