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Fig. 1 - Supplemental 2 Preparation of mouse anti-Ctla-4 antibody. (A) SDS-PAGE detection of the recombinant Cytotoxic T lymphocyte antigen-4 (Ctla-4) protein with extracellular domain (ECD). Lane 1, 2, and 3 represent the protein markers, blank, and target protein, respectively. (B) Western blot analysis of the mouse anti-EGFP and anti-Ctla-4 antibodies that bind to the recombinant Ctla-4-EGFP fusion proteins expressed in HEK293T cells. (C) Western blot analysis of native Ctla-4 protein in zebrafish intestinal tissues using mouse anti-Ctla-4 antibody.
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