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Fig. 5 - Supplemental 1 Examination on the activation of T cell subsets in the intestines of ctla-4-/- zebrafish. (A) Marker gene expression in individual cluster identifies the cluster as NKT-like cells. (B) RT-qPCR confirms the mRNA expression levels of Th2 cell marker genes in the intestines of wild-type (WT) and ctla-4-/- zebrafish. (C) RT-qPCR validates the mRNA expression levels of NKT-like cell marker genes in the intestines of WT and ctla-4-/- zebrafish. (D) Heatmap illustrates up-regulated genes involved in cytokine-cytokine receptor interaction in NKT-like cells from WT and ctla-4-/- samples. (E) KEGG enrichment analysis reveals the top 15 terms of up-regulated genes in Cd8+ T cells in ctla-4-/- samples versus WT samples. (F) Heatmap displays up-regulated genes involved in cytokine-cytokine receptor interaction in Cd8+ T cells from WT and ctla-4-/- samples. Statistical significance was assessed through an unpaired Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).