Fig. 1

Fig. 1 CellCousin-enabled partial ablation of hepatocytes and subsequent tracking of hepatocyte regeneration.

A Schematic illustrating CellCousin construct for labeling hepatocytes by Cre/lox-mediated-recombination. B Strategy for the ablation of mCherry-NTR-expressing hepatocytes. C Schematic illustration showing the two potential modes of hepatocyte regeneration after partial ablation and the associated fluorescent labeling. D Schematic illustrating the experimental strategy. E Longitudinal in vivo confocal imaging of the same liver at 0, 1 and 2 dppa showing emergence of mTagBFP2+ hepatocytes in presence of spared H2B-mGL+ hepatocytes. Scale bar: 200 μm. F Barplots with mean ± SD of the percentage of hepatocyte labeling before (pre-Mtz, n = 5 animals) and after Mtz treatment (0,1 and 5 dppa, n = 10 animals each). G Min-to-max boxplot showing quantification of normalized number of mTagBFP2+ cells before and after Mtz treatment (pre-Mtz, n = 14 animals; after Mtz treatment (0,1 and 5 dppa), n = 10 animals each) (Kruskal Wallis test).