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Fig. 1 Amh is required for the regulation of the circadian clock.Amh is required for the regulation of the circadian clock.The locomotor activities were monitored and analysed in female WT control and amh−/− mutants under LD (A–E) and DD (F–J) conditions. The total locomotor activities in 72 h (B, G), the quantification of average activities (C, H) and the moving distance and activity during the light-phase (D) dark-phase (E) subjective day (I) or subjective night (J) were shown. In the waveforms of locomotor activities, each point was calculated from the 5 min binned data across 3 days. For violin plots, dots and triangle correspond to individual zebrafish and the black and grey horizontal lines display the median as well as 25th and 75th percentiles. The box-and-whisker plots were presented as interquartile ranges with the median indicated by a line and whiskers extending from the minimum to the maximum values. For B–E n = 12 (WT) and n = 8 (amh−/−), (G–J) n = 8 (WT) and n = 24 (amh−/−). Statistical analysis was performed using two-sided unpaired t-test. K Quantitative real-time PCR analysis of core clock genes in pituitaries harvested at the seven indicated time points under DD condition from WT and amh−/− females. Target gene expression levels are shown relative to the beta-actin 2 reference gene actb2. The expression of WT CT2 was used for normalization. Data are presented as mean values ± SD, n = 3, two-sided unpaired t-test. ZT represents zeitgeber time, where ZT 0 corresponds to lights on; CT represents circadian time. Source data are provided as a Source Data file.