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Fig. 3 Mutation of the trpa1b gene affects the number of primordial germ cells (PGCs). (a) Distribution of PGCs in zebrafish with different genotypes. TG1, ihb327Tg. TG2, homozygous mutant offspring of trpa1b−/− crossed with TG1. (b) Quantification of PGC numbers in different strains. The numbers of samples were 27 and 56 in the TG1 and TG2 lines, respectively. *p < 0.05. (c) Protein expression of Ddx4 in testis of 6mpf zebrafish. KO1, germline tagged trpa1b−/− strains. KO2, germline unlabeled trpa1b−/− strains.
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Full text @ J. Fish Biol.