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Fig. 1 Expression of prep in regenerating livers. (A) Whole-mount in situ hybridization (WISH) images showing the expression of prep in the regenerating livers from the Tg (lfabp: GFP)+MTZ negative control group and Tg (lfabp: DenNTR)+MTZ group at BT, R0h, R8h, R24h and R48h; the arrow indicates the liver. (B) qPCR assay showing relative prep expression in the livers of the Tg (lfabp: DenNTR) and Tg (lfabp: DsRed) lines at BT, R0h, R8h, R24h and R48h. (C) Prep fluorescence in situ hybridization (FISH) and anti-2F11 staining images showing the regenerating livers at BT, R0h, R8h and R24h. **P<0.01, ***P<0.001 (two-tailed post hoc test). Data are mean±s.e.m. Scale bars: 100 μm (A); 75 µm (C, BT); 50 µm (C, 0h, R8h, R24h).