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Moore et al., 2024 - BMP signaling pathway member expression is enriched in enteric neural progenitors and required for zebrafish enteric nervous system development

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Fig. 4 p-SMAD-1/5/8 is present in Phox2b+ enteric progenitors during early phases of enteric development. Schematic of regions of interest in the zebrafish embryo (A), and timepoints assayed for p-SMAD-1/5/8 and enteric neuronal differentiation markers (B). Whole-mount Immunohistochemical analysis of activated BMP signaling by immunostaining of p-Smad-1/5/8 and neurogenesis marker Phox2b at 24 (C–E′), 36 (F–H′), 48 (I–K′), and 72 hpf (L–N′). Boxed region of co-expression of Phox2b and P-Smad1/5/8 (C′, F′, J′, M′). Anterior oriented to the left and dorsal to the top, in these images. Scale bar depicts 50 microns in C, F, I, and L. P.o.D., Post-otic Domain. ≥3 experiments with three biological replicates.

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