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Fig. 1 - Supplement 1 Adrb1-/- zebrafish embryos displayed a decrease of heart rate and blood flow in CVP. (A) adrb1-/- embryos displayed a decrease of heart rate compared to wild type embryos at 28hpf. The heartbeats of 26 adrb1-/- and 25 wild type embryos were counted over a 15-second period. Unpaired two-tailed t test was performed and p<0.0001. (B) adrb1-/- embryos showed significant decrease of RBC velocity compared to wild type embryos. Time-lapses on a single z-plane was performed at the frequency of 160.97ms/frame (372 frames/minute) on Fast Airyscanning. 10 embryos from each group were scanned, and 3 red blood cells were traced from each embryo. The measurement was performed using ImageJ. Unpaired two-tailed t-test, p=0.0346.