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Fig. 3 - Supplemental 7 Depletion of aqp1a.1 and aqp8a.1 expression alters vessel diameter. (A) Quantification of vessel diameter in wildtype and aquaporin mutant embryos at 2 dpf (wildtype: n = 178 aISVs/148 vISVs from 31 embryos; aqp1a.1rk28/rk28: n = 63 aISVs/42 vISVs from 21 embryos; aqp8a.1rk29/rk29: n = 39 aISVs/59 vISVs from 10 embryos; aqp1a.1rk28/rk28;aqp8a.1rk29/rk29: n = 36 aISVs/25 vISVs from 13 embryos). Data collected from three (wildtype) and two (aquaporin mutants) independent experiments. (B) Quantification of vessel diameter in Tg(fli1:myr-mCherry)ncv1 (n = 49 aISVs/32 vISVs from eight embryos), Tg(fli1ep:aqp1a.1-mEmerald)rk30 (n = 53 aISVs/58 vISVs from 15 embryos) and Tg(fli1ep:aqp8a.1-mEmerald)rk31 (n = 56 aISVs/43 vISVs from 12 embryos) transgenic embryos at 2 dpf. Data collected from two independent experiments. Statistical significance was determined by ordinary one-way ANOVA with Sidak’s multiple-comparison test; data is presented as mean ± SD. ns, p>0.05, *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. aISV, arterial intersegmental vessel; vISV, venous intersegmental vessel.