Fig. 2

Fig. 2 Inactivation of Wnt2bb impairs chondrocyte precursor proliferation. A and C: Single-plane and merged z-stack images of WT and wnt2bb−/− embryos with a Tg(fli1:EGFP) background at 36 hpf (A) and 48 hpf (C). Embryos were fixed and stained with anti-GFP antibodies. DAPI was used to label nuclei. Imaris software was applied to generate 3D volumes of pharyngeal arches 1 and 2, as well as virtual puncta representing cells in pharyngeal arches 1 and 2. B and D: Quantification of cell numbers in pharyngeal arches 1 and 2 of WT and wnt2bb−/− embryos at 36 hpf and 48 hpf using Imaris software (n = 6). E: Cell proliferation assays in WT and wnt2bb−/− embryos with a Tg(fli1:EGFP) background at 36 hpf (E). Virtual puncta represent BrdU incorporated cells in pharyngeal arches 1 and 2. F: Quantification of BrdU incorporated chondrogenic precursors in E (n = 3). ∗∗∗, P < 0.001 (student's t-test). WT, wild-type; PA, pharyngeal arch; hpf, hours post fertilization. Scale bar, 20 μm (A, C, E).