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Fig. 3 Expression of β-cat*-EGFP in NPCs promotes Wnt/β-catenin transcriptional activation. (a) Representative images of gfap:rtTA, TRE:β-cat*-EGFP, TCF:NLS-mCherry larvae or wild type siblings (Con.) at 3 dpf, treated with indicated Dox concentrations. (b, c) Quantification of mCherry fluorescence intensity within the CNS (b) and outside of the CNS (c) of larvae shown in (a) (n = 10 for all conditions). p-values were calculated for comparisons of all groups using the parametric one-way analysis of variance (ANOVA) with Tukey HSD post hoc test (no label = not significant). p-values directly above a bar indicate significant difference to all other groups by at least the level shown. Data are shown as mean ± standard deviation. (d) Representative lateral images of whole-mount in situ hybridization against axin2 transcript in gfap:rtTA, TRE:β-cat*-EGFP larvae at 3 dpf treated with indicated Dox concentrations (representative from n > 10 for all treatments). (e) Quantification of axin2 expression by qPCR. p-value was calculated using the parametric two-tailed t-test. Data are shown as mean ± standard deviation.