ZFIN Image: Ahmad et al., 2025, Fig 5

Image

Figure Caption

Fig 5 Asz1 is essential for piRNA granule organization ad repression of transposon expression.

A. 3 wpf asz1^+/+;Tg(ddx4:GFP) and asz1^-/-;Tg(ddx4:GFP) gonads are labeled for Ddx4 (green), Ziwi (red), ad DAPI (blue). Images are: top - merge images, middle - Ziwi channel, bottom - zoom-in images of white dashed boxes. Normal Ziwi localization to perinuclear granules is shown in asz1^+/+;Tg(ddx4:GFP) gonads. asz1^-/-;Tg(ddx4:GFP) gonads exhibit mostly undetected Ziwi granules (left), or over-aggregated and/or cytoplasmic Ziwi localization (right). Scale bars are 15 μm. B. Frequencies of Ziwi phenotypes from A are plotted. n = number of germ cells. Bars are mean ± SD. C. RT-qPCR analysis of the expression of germline transposons in 5 wpf gonads from each genotype (n = 50 gonads per genotype), as normalized for RNA loading by β-act as well as for germ cells by vasa expression (see methods). Bars are mean ± SD from biological duplicates, each made with technical replicates. Statistical analyses were tested by ANOVA.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.