Fig. 4

Fig. 4

Knock-down of candidate genes influenced EC proliferation in ISVs with and without blood flow. Embryos expressing Tg(fli1a:nls-mCherry) with a gene-specific or non-targeting control MO. Some embryos were co-injected with tnnt2a-MO to prevent blood flow (no flow), whereas controls did not receive tnnt2a-MO (flow). (A) Representative images showing a dividing EC (arrows) in a wnk1a MO-treated embryo under no flow conditions. (B) Proliferation was quantified from 54 to 70 hpf in a minimum of eight embryos per condition. The rate of EC proliferation in the ISVs was quantified as a percentage of nuclei dividing per hour. Data are presented as violin plots. Differences between means were analysed by two-way ANOVA. For the no-flow condition, only significant differences are shown, with non-significant differences omitted. In the flow condition, no statistically significant differences were detected.