Figure 1

Figure 1

Zebrafish fam83fa is expressed in the hatching gland (a) qRT-PCR of fam83fa expression (arbitrary units) in whole embryos across developmental time-series as shown. Data points = one biological replicate, n = 3. Error bars = s.e.m. All data normalized to 18S and calculated using the ∆∆Ct (Livak) method. (b–f) fam83fa expression by whole mount in situ hybridization in stages as labelled. Lateral views (b–f), dorsal (b) or ventral views (c′–f′) and zoomed in regions of centre image (b″–f″) shown. Numbers of embryos are denoted in first image. fam83fa is expressed in the pre-polster, the polster and the hatching gland. Scale bars = 250 μm (except 24 hpf = 500 μm). (g–i) hgg1 expression by whole mount in situ hybridization in stages as labelled. Lateral views (g–i), anterior (g’) or ventral views (h′–i’) and zoomed in regions of centre image (g″–f″) shown. Scale bars = 500 μm.