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Fig. 6 ubi:EGFPtyr transgenic fish exhibit robust and ubiquitous EGFP expression. (A) Bright-field and fluorescence images of ubi:EGFPtyr embryos versus wide-type (WT) at various developmental stages as indicated. Each panel contains an ubi:EGFPtyr embryo on the left and a WT embryo on the right. hpf, hours post fertilization; dpf, days post fertilization. (B) Histology sections of ubi:EGFPtyr adult fish stained with H&E or anti-EGFP antibody (IHC) demonstrating widespread but varying levels of EGFP expression among different tissue and cell types. All images were acquired under 400X magnification. (C) Representative flow cytometry analysis of the wild-type and ubi:EGFPtyr adult whole kidney marrow (WKM). Major blood lineages in WKM were resolved and identified as previously shown (left panels).36 EGFP expression was analyzed in each blood cell gate (middle and right panels). Gray, wild-type; pink, ubi:EGFPtyr.