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Fig. 3 Transient fibrotic deposition during liver regeneration after cryoinjury. (A-H) AFOG staining in sections of representative sham-operated (A,G) or injured livers (B-F,H) at the indicated stages. Blue indicates collagen; red indicates cell debris and fibrin. Anterior is towards the left; dorsal is towards the top. Outlined areas are shown at higher magnification. (G′,H′) Adjacent sections from the samples showed in G and H, immunostained using an anti-Col1a1 antibody and counterstained with DAPI. Asterisks indicate Col1a1 deposition in the liver parenchyma. (G″-H‴). Pseudo-colored Col1a1 signal (white) from G’ and H’. Magnifications of the outlined areas are shown in G‴ and H‴. Red asterisks indicate Col1a1 accumulation. (I) Quantification of the collagen area of livers from the indicated cohorts, normalized to the liver parenchyma area (n=6, 5, 6, 6 and 4, left to right). Data are mean±s.d. P-values were calculated using one-way ANOVA followed by Tukey's multiple comparisons test (**P<0.01, ****P<0.0001). bv, blood vessel; fc, fibrotic cap; IA, injured area; int, intestine; p, pancreas; vl, ventral lobe. Scale bars: 100 µm (white); 500 µm (orange and black)