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Fig. 1 Liver cell death following cryoinjury. (A-D) Simplified schematics illustrating the cryoinjury procedure in the zebrafish liver. (A) The zebrafish liver is placed ventral side up to facilitate the surgery. (B) A small incision near the midline exposes the ventral liver lobe. (C) The frozen cryoprobe is applied to the liver surface for 15 s to induce injury. (D) The damaged area in the liver appears as a blister-like structure at 1 dpci. (E,F) TUNEL-staining of sham-operated (E,E′) and injured (F,F′) liver sections at 1 dpci. IA, injured area. Yellow arrowheads indicate TUNEL+ cells; yellow dashed lines indicate the border zone. (G-J) Tg(fabp10a: GreenLantern-H2B; annexinV:mKate) in toto acquisitions of sham-operated (G,H) and 1 dpci (I,J) livers. (I,J) The IA (yellow dashed line) is identifiable by the absence of GreenLantern-H2B+ hepatocytes. Blue arrowheads indicate AnnexinV-mKate+ cells. Scale bars: 500 µm.