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Fig. 8 The protective effect and mechanism of QXY on ISO-induced cytoskeleton reorganization and hypertrophy in H9c2 cells. (A) The H9c2 cells were treated with ISO (100 µM), with or without QXY (3 µg/ml, 5 µg/ml), for 24 h. The intracellular cytoskeleton and nuclei were stained with Actin-Tracker Red-Rhodamine and DAPI, respectively. The protein concentration of cells was measured by BCA analysis. (B-C) The H9c2 cells were treated with ISO (100 µM), with or without QXY (5 µg/ml), for 24 h. The protein expression levels of phosphor-SMAD2/3, SMAD2, α-SMA, Vimentin, RhoA, ROCK1, phosphor-MYPT1, MYPT1, phosphor-MLC, and MLC were detected by western blotting. GAPDH served as the internal control. All the data were presented as mean ± SEM. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs control (Ctrl) group; *p < 0.05 and **p < 0.01 vs ISO-treated group. n = 4–6.
Reprinted from Phytomedicine : international journal of phytotherapy and phytopharmacology, 130, Zhou, Z.Y., Ma, J., Zhao, W.R., Shi, W.T., Zhang, J., Hu, Y.Y., Yue, M.Y., Zhou, W.L., Yan, H., Tang, J.Y., Wang, Y., Qiangxinyin formula protects against isoproterenol-induced cardiac hypertrophy, 155717155717, Copyright (2024) with permission from Elsevier. Full text @ Phytomedicine