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Fig. 6 The protective effect of icaritin (ICT) on ISO-induced cardiac hypertrophy and dysfunction in zebrafish. (A) The demonstration of ICA catalyzed to ICT by hydrolysis of the glucose groups. (B) The survival curve of 24 hpf zebrafish WT embryos treated with various concentrations (10, 30, 100, 300 and 1000 µM) of ICT for 5 days. (C) The pictures demonstrated the end-diastolic and systolic image of ventricle in various treatment groups. (D) Twenty-four hpf zebrafish Tg(cmlc2: GFP) embryos were treated with ISO (1 mM), with or without various concentrations (10, 30 and 100 µM) of ICT, for 48 h, then the cardiac morphology were observed by an inverted fluorescence microscope as well as the calculation of heart rate, cardiac output, stroke volume, EF, FS, SVED, SVES, ∆S, VVED and VVES. All the data were presented as mean ± SEM. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs control (Ctrl) group; *p < 0.05 and **p < 0.01 vs ISO-treated group. n = 4–6.
Reprinted from Phytomedicine : international journal of phytotherapy and phytopharmacology, 130, Zhou, Z.Y., Ma, J., Zhao, W.R., Shi, W.T., Zhang, J., Hu, Y.Y., Yue, M.Y., Zhou, W.L., Yan, H., Tang, J.Y., Wang, Y., Qiangxinyin formula protects against isoproterenol-induced cardiac hypertrophy, 155717155717, Copyright (2024) with permission from Elsevier. Full text @ Phytomedicine