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Fig. 6 Effects of 30 d of dietary treatments on histological characteristics of skeletal muscle in juvenile zebrafish. (a) Zebrafish whole-body transverse sections were obtained at the vent level and stained with wheat germ agglutinin (green, for plasma membrane). (b) Representative images of whole-body transverse sections, and the corresponding zoom-in images for white muscle fibres. The area of white muscle within the whole-body transverse section is presented in the image obtained from zebrafish fed the ASe. WM, white muscle; SC, spinal cord; NC, notochord; V, vent. Scale bar for whole-body transverse section, 500 μm; scale bar for white muscle fibre, 100 μm. Images were subjected to a morphological survey to calculate (c) the total cross-sectional area of white muscle and (d) the mean diameter of white muscle fibres. Values are means ± SEM, n 3. *Significantly different (P < 0·05, two-way (dietary Se× dietary VE or MHY1485) ANOVA followed by Bonferroni–Dunn multiple comparison). ASe, the basal Se-adequate diet; DSe, the basal Se-deficient diet; VE, DL-α-tocopherol acetate, antioxidant. MHY1485, activator of the target of rapamycin.