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Fig. 3 Effects of 30 d of dietary treatments on the levels of (a) ROS and (b) oxidative status biomarkers in skeletal muscle of juvenile zebrafish. (a) Three different ROS fluorescent probes (DCFH-DA, DHE and Amplex red) were applied to detect non-specific ROS, superoxide anion and hydrogen peroxide, respectively. (b) The levels of protein carbonyl and malondialdehyde were detected to evaluate oxidative status. Values are means ± SEM, n 3. *Significantly different, ns represents not significantly different (P < 0·05, two-way (dietary Se × dietary VE or MHY1485) ANOVA followed by Bonferroni-Dunn multiple comparison). ASe, the basal Se-adequate diet; DSe, the basal Se-deficient diet; VE, DL-α-tocopherol acetate, antioxidant; MHY1485, activator of the target of rapamycin; ROS, reactive oxygen species; DCFH-DA, 2’,7’-dichlorodihydrofluorescein diacetate; DHE, dihydroethidium; MDA, malondialdehyde.