Fig. 2

Fig. 2 Temperature-induced differential FOXO1-DNA binding and transcriptomic changes in H1 ESCs.

a Left: fold change vs. -log₁₀ FDR-corrected Q-value for differential FOXO1-binding at 37 °C or 4 °C 4 h in H1 ESCs revealed by CUT&Tag-seq; right: IGV (Integrative Genomics Viewer) snapshots showing FOXO1-binding signals around the DHX9 and EP300 loci (right); kb, kilobase pairs. b Line plots summarizing the mean of FOXO1 CUT&Tag signals within 3 kb up- and down-stream of gene transcription start sites (TSS) in H1 ESCs at 37 °C and 4 °C 4 h; kb, kilobase pairs. c Venn diagram indicating the overlap between differentially expressed genes (DEGs) upon rewarming and 4 °C-induced FOXO1 differentially bound genes in H1 ESCs. d Enrichment analysis on the overlapped genes from (c); left: Enrichment analysis on genes that had higher levels of transcripts at 4 °C 4 h compared to 4 °C 4 h_37 °C 2 h (rewarmed); right: Enrichment analysis on genes that had higher levels of transcripts at the rewarmed stage compared to 4 °C 4 h. P values in (d) were calculated in Metascape.