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Fig. 3 Conjugated CDs deliver cargo to bones. Caudal fins of zebrafish injected 4 days after amputation with (A) PBS, (B) CD, (C) CD-Cy5, (D) CD-Biotin, (E) CD and Streptavidin-Alexa 647, and (F) CD-Biotin and Streptavidin-Alexa 647 and imaging the following day. Deposition of CDs and cargo (Cy5 or Streptaviding-Alexa-647) was observed at 488 and 647 nm, respectively. Anterior is to the right and dorsal to the top. Arrows indicate areas of cargo deposition, and dashed lines are the planes of histological sectioning. Scale bar is 500 μm. (A’–F’) Cryosection images were observed under 360 nm (DAPI, nuclei), 488 nm (CD), and 647 nm (cargo) excitation light. Arrows indicate the areas of cargo deposition. BV is a blood vessel that shows blood autofluorescence. Scale bar is 50 μm.