IMAGE

Fig. 7

ID
ZDB-IMAGE-240320-2
Genes
Antibodies
Source
Figures for She et al., 2020
Image
Figure Caption

Fig. 7 Grl-Smyd2 mediates Stat3 activation during heart regeneration. (A-D) Immunofluorescent images of injured ventricles stained with anti-P-Stat3 antibody (green) and anti-Mef2 antibody (red) from WT siblings (A), grl5nt−/− mutant fish (B), 4-HT-treated Tg(cmlc2:nRSGG) control animals (C) and 4-HT-treated Tg(cmlc2:creER;cmlc2:nRGG) animals (D) at 7 dpa. Insets show higher-magnification images of the dashed boxes. Arrowheads indicate P-Stat3 overlapping with Mef2 in CM nuclei; Arrows indicate perinuclear cytosolic P-Stat3 around Mef2 nuclei. P-Stat3 immunostaining was also detectable in non-CM cells in the injured area. (E) Quantification of number of P-Stat3-positive CMs in the injury border zone of 7 dpa ventricles from WT siblings (n=5), grl5nt−/− mutants (n=4), Tg(cmlc2:nRSGG) control fish (n=5), or Tg(cmlc2:creER;cmlc2:nRGG) fish (n=6). (F-H) Immunofluorescent section images of injured ventricles stained with anti-P-Stat3 antibody (green) and anti-Mef2 antibody (red) from DMSO-treated (F), AZ505-treated (G) and LLY-507-treated grl5nt−/− mutant fish (H) at 7 dpa. Insets show higher-magnification images of the dashed boxes. Arrowheads indicate P-Stat3 overlapping with Mef2; Arrows indicate perinuclear cytosolic P-Stat3 around Mef2 nuclei. (I) Quantification of number of P-Stat3-positive CMs in injury border zones at 7 dpa from DMSO-treated (n=6), AZ505-treated (n=4) and LLY-507-treated grl5nt−/− mutant fish (n=4). (J) qPCR analyses of bcl2a, ccnd1 and socs3b in injured hearts extracted from Tg(cmlc2:creER;cmlc2:nRSGG) and Tg(cmlc2:nRSGG) control fish, as well as WT sibling and grl5nt−/− mutant fish (n=3). Data presents as mean±s.e.m. **P<0.01, ***P<0.001, ****P<0.0001, Student's t-test (unpaired, two-tailed). Scale bars: 100 µm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development