Fig. 7

Fig. 7

Signs of increased apoptosis in spermidine treated zebrafish larvae. (A) Non-transgenic (WTTAB) zebrafish larvae aged 2 days post fertlisation (dpf) showed increased acridine orange apoptosis staining following spermidine treatment (p = 0.0054). The increase in acridine orange staining in spermidine treated Ubb-Ataxin-3 84Q BFP zebrafish larvae was not statistically significant (p > 0.0644). (B) Comparison of the motor behaviour (total distance swum) of WT TAB zebrafish larvae treated with Vehicle or Spermidine found no difference between the groups. (C) Immunoblot analysis was performed to examine signs of apoptosis in non-transgenic zebrafish following spermidine treatment. (D) Spermidine treatment resulted in increased levels of cleaved PARP and (E) cleaved caspase 3, both signs of apoptosis (p = 0.0196 and p = 0.0255 respectively, n = 7). Statistical analysis performed were a two-way ANOVA followed by a Tukey post-hoc analysis and a paired Student t-test. Data represents mean ± SEM. Data points reflected are experimental treatments of approximately 20–25 larvae