Fig. 1

Fig. 1 Effect of neurog1hi1059/hi1059 and olig2m1317/m1317 mutants on dopaminergic development (A-F) th expression in (B,D,F) neurog1hi1059/hi1059 mutants compared to (A,C,E) wildtype siblings at (A,B) 30 hpf and (C–F) 72 hpf. (A,B) At 30 hpf in neurog1hi1059/hi1059 mutants no th+ cells are present (black arrowheads). (C–F) In the H/PT area, DAC2, DAC4 and DAC5 are missing (black arrowheads) and DAC6 is reduced (non-filled arrowhead). (G–L) th expression in (H,J,L) olig2m1317/m1317 mutants compared to (G,I,K) wildtype siblings at (G,H) 30 hpf and (I–L) 72 hpf. At 30 hpf in (H) olig2m1317/m1317 mutants, the number of th+ cells is reduced compared to (G) wildtype (non-filled arrowhead). At 72 hpf in (J,L) olig2m1317/m1317 mutants the numbers of th+ DAC2 (non-filled arrowheads) and DAC6 (black arrowheads) cells are reduced compared to (I,K) wildtype siblings. (M,N) Quantification of th+ cells in mutant neurog1 (M) and olig2 (N) embryos compared to wildtype siblings at 72 hpf. The number (n) of embryos analyzed for the cell counts are as indicated in the grey box; p-values were calculated using the Mann-Whitney test. p-values indicate significance if they are <0.05 (**, 0.001–0.01; ***, 0.0001–0.001; ****, <0.0001), if no asterisks are present, effects are not significant. For numbers see Supplemental Table 1. Abbreviations: numbers 1 through 6 for DAC1-6, dopaminergic cell cluster 1–6; H, hypothalamus; LC, locus coeruleus; MO, medulla oblongata; OB, olfactory bulb; PO, preoptic area; Pr, pretectum; PT, posterior tuberculum; SP, subpallium; TC, telencephalic cluster. (A-D, G-J) lateral views (enucleated embryos: C, D and I, J) and (E, F, K, L) dorsal views. All images show single optical planes. Anterior is to the left. Scale bars: 100 μm.