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Fig. 1 Developmental effects of zebrafish embryos exposure to echimidine. (A) Death rate from 12 to 96 hpf every 24 h. (B) Hatching rate at 48 and 52 – 72 hpf every 2 h. (C) Embryonic movement at 24 hpf; (D) Morphological changes were detected by a microscope. Scale bar: 500 µm. (E) Body length at 96 hpf. The data of death rate and hatching rate were analyzed by two-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test and presented as the mean ± SEM (n = 6). Other data were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s multiple comparisons test and presented as the mean ± SEM (n ≥ 25). * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001.