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Fig. 4 (A) Structure of the chromogenic β-lactamase substrate nitrocefin (10), which is hydrolyzed to 11 following β-lactamase catalysis. (B) Western blot analysis of β-lactamase expression in embryos injected with 400 pg of mRNA. (C) Zebrafish lysates analyzed for β-lactamase activity by monitoring production of 11 (absorbance at 486 nm) over time. (D) Initial velocities were determined through a linear regression analysis of the first 10 min of the data presented in (C). The error values indicate the standard deviation of values for the best fit parameters from the linear regression analysis.(A) Structure of the chromogenic β-lactamase substrate nitrocefin (10), which is hydrolyzed to 11 following β-lactamase catalysis. (B) Western blot analysis of β-lactamase expression in embryos injected with 400 pg of mRNA. (C) Zebrafish lysates analyzed for β-lactamase activity by monitoring production of 11 (absorbance at 486 nm) over time. (D) Initial velocities were determined through a linear regression analysis of the first 10 min of the data presented in (C). The error values indicate the standard deviation of values for the best fit parameters from the linear regression analysis.