Fig. 9.

Fig. 9.

p38-mediated BMP signalling does not differ in chondrocytes of heat-shocked, dnBMPR zebrafish embryos. (A-D′) Condensing ceratohyal mesenchyme did not show much p-p38 immunoreactivity at 48 hpf in non-heat-shocked wild-type (A,A′) and dnBMPR (B,B′) embryos and heat-shocked wild-type (C,C′) and dnBMPR (D,D′) embryos (i.e. 24 h after 20-min heat shock). (E) Quantitative image analyses (n=3 for each group) revealed no significant differences in p-p38 levels in condensing ceratohyal mesenchyme among experimental groups. One-way ANOVA and paired Student's t-test. (F-I′) Chondrocytes of heat-shocked dnBMPR zebrafish embryos (I,I′) showed similar levels of p-p38 immunoreactivity at 72 hpf (i.e. 48 h after heat shock) as control groups (F-H′). (J) Quantitative image analyses (n=3 for each group) revealed no significant differences in p-p38 levels in chondrocytes of heat-shocked dnBMPR zebrafish, compared with each control group. One-way ANOVA and paired Student's t-test. Scale bars: 50 μm. a.u., arbitrary units; HS, heat-shocked; wt, wild type.