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Publication: Yamamoto et al., 2024 - Trpv4-mediated apoptosis of Leydig cells induced by high temperature regulates sperm development and motility in zebrafish
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Fig. 2 Higher temperature led to upregulated expression of Trpv4 in Leydig cells.

a qRT-PCR analysis of Trp family genes in the testes. Values are the mean ± SEM (n = 3). **p < 0.01 and *p < 0.05 compared with the 29 °C testis. b In situ hybridization of Trpv4 in testes. c Double in situ hybridization of Trpv4 (blue) and Insl3 (yellow). The insets were magnified in the bottom rows. Scale bars are 20 μm (b and c: high magnification) and 50 μm (c: low magnification).

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